Elevated serum microRNA 483-5p levels may predict patients at risk of post-operative atrial fibrillation

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Title: Elevated serum microRNA 483-5p levels may predict patients at risk of post-operative atrial fibrillation
Authors: Harling, L
Lambert, J
Ashrafian, H
Darzi, A
Gooderham, N
Athanasiou, T
Item Type: Journal Article
Abstract: Objective: Atrial fibrillation (POAF) is the commonest post-operative cardiac arrhythmia, affecting approximately 1 in 3 patients undergoing coronary artery bypass grafting (CABG). Although its aetiology is complex, atrial substrate changes may pre-dispose to its onset. This study aims to ascertain the atrial microRNA signature of POAF and determine the potential for circulating microRNA as a pre-operative biomarker for this arrhythmia. Methods: 34 patients undergoing non-emergent, on-pump CABG were prospectively recruited. Right atrial biopsies were taken intra-operatively and snap frozen for RNA extraction. Plasma was obtained at 24 hours pre-operatively and at 2 and 4-days post-operatively. POAF was defined by continuous Holter recording. Inter-group comparisons were performed using student t-test or ANOVA as required. Receiver operating characteristics (ROC) analysis was used to determine the diagnostic accuracy of pre-operative serum miRNA as a POAF biomarker. Results: 16 microRNA were differentially expressed in the atrial myocardium of POAF patients when compared to those maintaining sinus rhythm (SR). MiR-208a was the most under-expressed (FC:2.458) and miR-483-5p the most over-expressed (FC:1.804). Mir-483-5p also demonstrated significant overexpression in the pre-operative serum of these patients, with ROC analysis demonstrating an overall predictive accuracy of 78%. Conclusions: This study provides the first description of atrial myocardial and circulating plasma microRNA in POAF patients. Our findings suggest POAF may be associated with pre-existing atrial substrate differences predisposing to arrhythmogenesis. Moreover, this study highlights the potential for miR-483-5p in biomarker development. Further work must now perform prospective, targeted validation of these results in a larger patient cohort.
Issue Date: 15-Jul-2016
Date of Acceptance: 8-Jun-2016
ISSN: 1873-734X
Publisher: Oxford University Press (OUP)
Start Page: 73
End Page: 78
Journal / Book Title: European Journal of Cardio-thoracic Surgery
Volume: 51
Issue: 1
Copyright Statement: © The Author 2016. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Imperial College Healthcare Charity
Wellcome Trust
Funder's Grant Number: 5117/R20R
Keywords: Science & Technology
Life Sciences & Biomedicine
Cardiac & Cardiovascular Systems
Respiratory System
Cardiovascular System & Cardiology
Post-operative atrial fibrillation
Atrial Fibrillation
Coronary Artery Bypass
Follow-Up Studies
Heart Atria
Microarray Analysis
Middle Aged
Postoperative Complications
Prospective Studies
ROC Curve
Risk Factors
Coronary Artery Bypass Grafting
1102 Cardiovascular Medicine And Haematology
Publication Status: Published
Appears in Collections:Division of Surgery
Faculty of Medicine

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