Designing an Artificial Golgi Reactor to achieve targeted glycosylation of monoclonal antibodies

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Title: Designing an Artificial Golgi Reactor to achieve targeted glycosylation of monoclonal antibodies
Authors: Klymenko, O
Royle, K
Polizzi, KM
Shah, N
Kontoravdi, C
Item Type: Journal Article
Abstract: The therapeutic efficacy of monoclonal antibodies (mAbs) is dependent upon their glycosylation patterns. As the largest group of currently approved biopharmaceuticals, the microheterogeneity in mAb oligosaccharide profiles deriving from mammalian cell production is a challenge to the biopharmaceutical industry. Disengaging the glycosylation process from the cell may offer significant enhancement of product quality and allow better control and reproducibility in line with the Quality by Design paradigm. Three potential designs of an Artificial Golgi reactor implementing targeted sequential glycosylation of mAbs are proposed including a (i) microcapillary film reactor, (ii) packed bed reactor with non-porous pellets, and (iii) packed bed reactor with porous pellets. Detailed mathematical models are developed to predict their performance for a range of design and operational parameters. While all three reactor designs can achieve desired conversion levels, the choice of a particular one depends on the required throughput and the associated cost of enzymes and co-substrates.
Issue Date: 15-Jul-2016
Date of Acceptance: 21-Jun-2016
URI: http://hdl.handle.net/10044/1/34029
DOI: https://dx.doi.org/10.1002/aic.15388
ISSN: 0001-1541
Publisher: Wiley
Start Page: 2959
End Page: 2973
Journal / Book Title: AICHE Journal
Volume: 62
Issue: 9
Copyright Statement: Available via Open Access at http://onlinelibrary.wiley.com/doi/10.1002/aic.15388/abstract
Sponsor/Funder: Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: 09066
EP/K038648/1
Keywords: Chemical Engineering
0904 Chemical Engineering
0914 Resources Engineering And Extractive Metallurgy
Publication Status: Published
Appears in Collections:Faculty of Engineering
Centre for Environmental Policy
Chemical Engineering
Faculty of Natural Sciences



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