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Insoluble and soluble roasted walnut proteins retain antibody reactivity

Title: Insoluble and soluble roasted walnut proteins retain antibody reactivity
Authors: Downs, ML
Simpson, A
Custovic, A
Semic-Jusufagic, A
Bartra, J
Fernandez-Rivas, M
Taylor, SL
Baumert, JL
Mills, EN
Item Type: Journal Article
Abstract: Thermal processing techniques commonly used during food production have the potential to impact food allergens by inducing physical and/or chemical changes to the proteins. English walnuts (Juglans regia) are among the most commonly allergenic tree nuts, but little information is available regarding how walnut allergens respond to thermal processing. This study evaluated the effects of dry roasting (132 or 180°C for 5, 10, or 20min) on the solubility and immunoreactivity of walnut proteins. A dramatic decrease in walnut protein solubility was observed following dry roasting at 180°C for 20min. However, both the soluble and insoluble protein fractions from roasted walnuts maintained substantial amounts of IgG immunoreactivity (using anti-raw and anti-roasted walnut antisera), with similar patterns of reactivity observed for human IgE from walnut-allergic individuals. Thus, walnut proteins are relatively stable under certain thermal processing conditions, and IgE reactivity remains present even when insoluble aggregates are formed.
Issue Date: 29-Aug-2015
Date of Acceptance: 27-Aug-2015
URI: http://hdl.handle.net/10044/1/33271
DOI: http://dx.doi.org/10.1016/j.foodchem.2015.08.119
ISSN: 1873-7072
Publisher: Elsevier
Start Page: 1013
End Page: 1021
Journal / Book Title: Food Chemistry
Volume: 194
Copyright Statement: © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords: Food allergy
Thermal processing
Tree nut
Walnut
Allergens
Food Hypersensitivity
Immunoglobulin E
Juglans
Nuts
Plant Proteins
Food Science
MD Multidisciplinary
Publication Status: Published
Appears in Collections:Department of Medicine
Faculty of Medicine



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