A novel adhesion molecule in the murine thymic microenvironment: functional and biochemical analysis

File Description SizeFormat 
018016.pdfPublished version9.89 MBAdobe PDFDownload
Title: A novel adhesion molecule in the murine thymic microenvironment: functional and biochemical analysis
Author(s): Imami, N
Ladyman, HM
Spanopoulou, E
Ritter, MA
Item Type: Journal Article
Abstract: The rat monoclonal antibody (mAb) 4F1, raised against mouse thymic stromal cells, recognizes cortical epithelium in tissue sections of mouse thymus; however, in flow cytometry, activated leucocytes (T cells, B cells, and macrophages) and transformed thymocytes are also positive for the 4F1-antigen (4F1-Ag). Western blotting, under both reducing and nonreducing conditions, demonstrates that the molecule to which 4F1 binds is expressed in four forms, 29, 32, 40, and 43 kD, all of which carry N-linked carbohydrate; and that the structure is identical on epithelium and lymphocytes. The 4F1-Ag on cortical epithelium is partially sensitive to PI-PLC treatment, whereas on transformed epithelial and lymphoid cell lines, it was resistant to this enzyme. The molecule, therefore, may exist in both transmembrane and phosphoinositol-linked forms. In functional blocking experiments, mAb 4F1 gave inhibition of both T-cell proliferation in MLR and of cytotoxic T-cell killing of alloantigenic targets; it also blocked adhesion of transformed thymocytes to thymic epithelial cells in vitro. These molecular and functional characteristics suggest that the 4F1-Ag is a novel adhesion molecule that may be involved both in intrathymic T lymphocyte differentiation and in peripheral T-cell function. © 1992, Harwood Academic Publishers GmbH.
Publication Date: 31-Dec-1992
Date of Acceptance: 31-Dec-1992
URI: http://hdl.handle.net/10044/1/32983
DOI: http://dx.doi.org/10.1155/1992/18016
ISSN: 1044-6672
Publisher: Harwood Academic Publishers GmbH
Start Page: 161
End Page: 173
Journal / Book Title: Developmental Immunology
Volume: 2
Issue: 2
Copyright Statement: © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright Statement: © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Animals
Antibodies, Monoclonal
Cell Adhesion Molecules
Cell Division
Cell Line
Glycosylation
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Phosphatidylinositols
Spleen
Thymus Gland
Tunicamycin
Type C Phospholipases
Spleen
Thymus Gland
Cell Line
Animals
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Mice
Phosphatidylinositols
Cell Adhesion Molecules
Tunicamycin
Antibodies, Monoclonal
Cell Division
Glycosylation
Type C Phospholipases
Immunology
1107 Immunology
Publication Status: Published
Appears in Collections:Department of Medicine



Items in Spiral are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons