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Investigation of the Impact of Desorption Electrospray Ionization Sprayer Geometry on Its Performance in Imaging of Biological Tissue.

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Title: Investigation of the Impact of Desorption Electrospray Ionization Sprayer Geometry on Its Performance in Imaging of Biological Tissue.
Authors: Tillner, J
McKenzie, JS
Jones, EA
Speller, AV
Walsh, JL
Veselkov, KA
Bunch, J
Takats, Z
Gilmore, IS
Item Type: Journal Article
Abstract: In this study, the impact of sprayer design and geometry on performance in desorption electrospray ionization mass spectrometry (DESI-MS) is assessed, as the sprayer is thought to be a major source of variability. Absolute intensity repeatability, spectral composition, and classification accuracy for biological tissues are considered. Marked differences in tissue analysis performance are seen between the commercially available and a lab-built sprayer. These are thought to be associated with the geometry of the solvent capillary and the resulting shape of the primary electrospray. Experiments with a sprayer with a fixed solvent capillary position show that capillary orientation has a crucial impact on tissue complex lipid signal and can lead to an almost complete loss of signal. Absolute intensity repeatability is compared for five lab-built sprayers using pork liver sections. Repeatability ranges from 1 to 224% for individual sprayers and peaks of different spectral abundance. Between sprayers, repeatability is 16%, 9%, 23%, and 34% for high, medium, low, and very low abundance peaks, respectively. To assess the impact of sprayer variability on tissue classification using multivariate statistical tools, nine human colorectal adenocarcinoma sections are analyzed with three lab-built sprayers, and classification accuracy for adenocarcinoma versus the surrounding stroma is assessed. It ranges from 80.7 to 94.5% between the three sprayers and is 86.5% overall. The presented results confirm that the sprayer setup needs to be closely controlled to obtain reliable data, and a new sprayer setup with a fixed solvent capillary geometry should be developed.
Issue Date: 25-Mar-2016
Date of Acceptance: 25-Mar-2016
URI: http://hdl.handle.net/10044/1/32459
DOI: https://dx.doi.org/10.1021/acs.analchem.6b00345
ISSN: 0003-2700
Publisher: ACS Publications
Start Page: 4808
End Page: 4816
Journal / Book Title: Analytical Chemistry
Volume: 88
Issue: 9
Copyright Statement: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://dx.doi.org/10.1021/acs.analchem.6b00345
Sponsor/Funder: Cystic Fibrosis Trust
National Physical Laboratory
Commission of the European Communities
Funder's Grant Number: SRC 001
410740
634402
Keywords: Analytical Chemistry
0301 Analytical Chemistry
0904 Chemical Engineering
0399 Other Chemical Sciences
Publication Status: Published
Appears in Collections:Division of Surgery
Faculty of Medicine



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