A computational model of gene expression in an inducible synthetic circuit

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Title: A computational model of gene expression in an inducible synthetic circuit
Author(s): Ceroni, F
Furini, S
Cavalcanti, S
Item Type: Conference Paper
Abstract: Synthetic biology aims to the rational design of gene circuits with predictable behaviours. Great efforts have been done so far to introduce in the field mathematical models that could facilitate the design of synthetic networks. Here we present a mathematical model of a synthetic gene-circuit with a negative feedback. The closed loop configuration allows the control of transcription by an inducer molecule (IPTG). Escherichia coli bacterial cells were transformed and expression of a fluorescent reporter (GFP) was measured for different inducer levels. Computer model simulations well reproduced the experimental induction data, using a single fitting parameter. Independent genetic components were used to assemble the synthetic circuit. The mathematical model here presented could be useful to predict how changes in these genetic components affect the behaviour of the synthetic circuit.
Publication Date: 1-Oct-2009
Date of Acceptance: 1-Oct-2009
URI: http://hdl.handle.net/10044/1/31353
DOI: http://dx.doi.org/10.1142/9789814295291_0043
ISSN: 2335-6936
Publisher: World Scientific Publishing Company
Start Page: 409
End Page: 420
Journal / Book Title: Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing
Copyright Statement: © 2009 World Scientific Publishing Company
Conference Name: Biocomputing 2010
Keywords: Computational Biology
Computer Simulation
Escherichia coli
Escherichia coli Proteins
Gene Expression
Gene Regulatory Networks
Genes, Reporter
Green Fluorescent Proteins
Lac Repressors
Models, Genetic
Promoter Regions, Genetic
Synthetic Biology
Escherichia coli
Escherichia coli Proteins
Green Fluorescent Proteins
Computational Biology
Gene Expression
Genes, Reporter
Models, Genetic
Computer Simulation
Gene Regulatory Networks
Promoter Regions, Genetic
Lac Repressors
Synthetic Biology
Publication Status: Published
Start Date: 2010-01-04
Finish Date: 2010-01-08
Conference Place: Kamuela, Hawaii, USA
Appears in Collections:Faculty of Engineering
Bioengineering



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