A protease-based biosensor for the detection of schistosome cercariae

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Title: A protease-based biosensor for the detection of schistosome cercariae
Author(s): Webb, AJ
Kelwick, R
Doenhoff, MJ
Kylilis, N
MacDonald, J
Wen, KY
Mckeown, C
Baldwin, G
Ellis, T
Jensen, K
Freemont, PS
Item Type: Journal Article
Abstract: Parasitic diseases affect millions of people worldwide, causing debilitating illnesses and death. Rapid and cost-effective approaches to detect parasites are needed, especially in resource-limited settings. A common signature of parasitic diseases is the release of specific proteases by the parasites at multiple stages during their life cycles. To this end, we engineered several modular Escherichia coli and Bacillus subtilis whole-cell-based biosensors which incorporate an interchangeable protease recognition motif into their designs. Herein, we describe how several of our engineered biosensors have been applied to detect the presence and activity of elastase, an enzyme released by the cercarial larvae stage of Schistosoma mansoni. Collectively, S. mansoni and several other schistosomes are responsible for the infection of an estimated 200 million people worldwide. Since our biosensors are maintained in lyophilised cells, they could be applied for the detection of S. mansoni and other parasites in settings without reliable cold chain access.
Publication Date: 19-Apr-2016
Date of Acceptance: 30-Mar-2016
URI: http://hdl.handle.net/10044/1/31012
DOI: https://dx.doi.org/10.1038/srep24725
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 6
Sponsor/Funder: Bill & Melinda Gates Foundation
Engineering & Physical Science Research Council (E
Funder's Grant Number: OPP1046311
EP/J02175X/1
Copyright Statement: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Publication Status: Published
Article Number: 24725
Appears in Collections:Faculty of Engineering
Bioengineering
Department of Medicine
Faculty of Medicine



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