A monoclonal antibody recognizing very late activation antigen-4 inhibits eosinophil accumulation in vivo.

Title: A monoclonal antibody recognizing very late activation antigen-4 inhibits eosinophil accumulation in vivo.
Author(s): Weg, VB
Williams, TJ
Lobb, RR
Nourshargh, S
Item Type: Journal Article
Abstract: Using an in vivo test system, the role of the β1 integrin very late activation antigen-4 (VLA-4) in eosinophil accumulation in allergic and nonallergic inflammatory reactions was investigated. Eosinophil infiltration and edema formation were measured as the local accumulation of intravenously injected 111In-labeled eosinophils and 125I-human serum albumin. The inflammatory reactions investigated were a passive cutaneous anaphylaxis (PCA) reaction and responses elicited by intradermal soluble inflammatory mediators (platelet-activating factor, leukotriene B4, C5a des Arg), arachidonic acid, and zymosan particles. The in vitro pretreatment of 111In-eosinophils with the anti-VLA-4 monoclonal antibody (mAb) HP1/2, which crossreacts with guinea pig eosinophils, suppressed eosinophil accumulation in all the inflammatory reactions investigated. Eosinophil accumulation was inhibited to the same extent when mAb HP1/2 was administered intravenously. It is interesting that HP1/2 had no effect on stimulated edema formation. These results suggest a role for VLA-4 in eosinophil accumulation in vivo and indicate a dissociation between the inflammatory events of eosinophil accumulation and edema formation.
Publication Date: 1-Feb-1993
URI: http://hdl.handle.net/10044/1/28131
ISSN: 0022-1007
Start Page: 561
End Page: 566
Journal / Book Title: Journal of Experimental Medicine
Volume: 177
Issue: 2
Copyright Statement: © The Rockefeller University Pres
Keywords: Animals
Antibodies, Monoclonal
Edema
Eosinophils
Guinea Pigs
Inflammation
Leukotriene B4
Passive Cutaneous Anaphylaxis
Platelet Activating Factor
Receptors, Very Late Antigen
Zymosan
Publication Status: Published
Appears in Collections:National Heart and Lung Institute
Airway Disease
Faculty of Medicine



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