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Gene-to-ecosystem impacts of a catastrophic pesticide spill: testing a multilevel bioassessment approach in a river ecosystem

Title: Gene-to-ecosystem impacts of a catastrophic pesticide spill: testing a multilevel bioassessment approach in a river ecosystem
Authors: Gray, CE
Thompson, M
Bankier, C
Bell, T
Dumbrell, A
Ledger, M
Lehmann, K
McKew, B
Sayer, C
Shelley, F
Trimmer, M
Warren, S
Woodward, G
Item Type: Journal Article
Abstract: 1.Pesticides can have strong deleterious impacts in fresh waters, but understanding how these effects cascade through natural ecosystems, from microbes to apex predators, is limited because research that spans multiple levels of biological organisation is rare. 2.We report how an accidental insecticide spill altered the structure and functioning of a river across levels ranging from genes to ecosystems. We quantified the impacts on assemblages of microbes, diatoms, macroinvertebrates and fish and measured leaf-litter decomposition rates and microbial functional potential at upstream control and downstream impacted sites 2 months after the spill. 3.Both direct and indirect impacts were evident across multiple levels of organisation and taxa, from the base of the food web to higher trophic levels. At the molecular level, differences in functional gene abundance within the impacted sites reflected a combination of direct and indirect effects of the pesticide, via elevated abundances of microbial populations capable of using chlorpyrifos as a resource (i.e. direct effect) and oxidising ammonia released by decaying macroinvertebrate carcasses (i.e. indirect effect). 4.At the base of the food chains, diatom taxa found only in the impacted sites were an order-of-magnitude larger in cell size than the largest comparable taxa in control communities, following the near extirpation of their consumers. Population biomass of the key detritivore Gammarus pulex was markedly lower, as was the rate of litter decomposition in the impacted sites. This was partially compensated for, however, by elevated microbial breakdown, suggesting another indirect food-web effect of the toxic spill. 5.Although many species exhibited population crashes or local extirpation, total macroinvertebrate biomass and abundance were largely unaffected due to a compensatory elevation in small tolerant taxa such as oligochaetes, and/or taxa which were in their adult aerial life stage at the time of the spill (e.g. chironomids) and thus avoided contact with the polluted waters and were able to repopulate the river quickly. Mass–abundance scaling of trophic links between consumers and resources revealed extensive restructuring within the food web. 6.This case study shows that pesticides can affect food-web structure and ecosystem functioning, both directly and indirectly across levels of biological organisation. It also demonstrates how an integrated assessment approach, as adopted here, can elucidate links between microbiota, macroinvertebrates and fish, for instance, thus improving our understanding of the range of biological consequences of chemical contamination in natural ecosystems.
Issue Date: 24-Sep-2015
Date of Acceptance: 24-Sep-2015
URI: http://hdl.handle.net/10044/1/27675
DOI: https://dx.doi.org/10.1111/fwb.12676
ISSN: 1365-2427
Publisher: Wiley
Start Page: 2037
End Page: 2050
Journal / Book Title: Freshwater Biology
Volume: 61
Issue: 12
Copyright Statement: © 2015 John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Thompson, M. S. A., Bankier, C., Bell, T., Dumbrell, A. J., Gray, C., Ledger, M. E., Lehmann, K., McKew, B. A., Sayer, C. D., Shelley, F., Trimmer, M., Warren, S. L. and Woodward, G. (2015), Gene-to-ecosystem impacts of a catastrophic pesticide spill: testing a multilevel bioassessment approach in a river ecosystem. Freshwater Biology. doi: 10.1111/fwb.12676, which has been published in final form at https://dx.doi.org/10.1111/fwb.12676. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving (http://olabout.wiley.com/WileyCDA/Section/id-820227.html#terms).
Sponsor/Funder: Natural Environment Research Council (NERC)
Funder's Grant Number: NE/L008491/1
Keywords: Marine Biology & Hydrobiology
05 Environmental Sciences
06 Biological Sciences
Publication Status: Published
Appears in Collections:Faculty of Natural Sciences



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