Differentiation of tuberculosis strains in a population with mainly Beijing-family strains.

Title: Differentiation of tuberculosis strains in a population with mainly Beijing-family strains.
Authors: Nikolayevskyy, V
Gopaul, K
Balabanova, Y
Brown, T
Fedorin, I
Drobniewski, F
Item Type: Journal Article
Abstract: A high prevalence of tuberculosis (TB) isolates that are genetically homogenous and from the Beijing family has been reported in Russia. To map TB transmission caused by these strains, new genotyping systems are needed. Mycobacterial interspersed repetitive units (MIRUs) offer the possibility of rapid PCR-based typing with comparable discrimination to IS6110 restriction fragment length polymorphism techniques. Spoligotyping and detection of IS6110 insertion in the dnaA-dnaN region were used to identify Beijing strains in 187 Mycobacterium tuberculosis isolates from Samara, Russia. The Beijing isolates were analyzed by using 12-MIRU and 3-exact tandem repeats (ETR) loci and by an expanded set of 10 additional variable number tandem repeats loci. The expanded set of 25 MIRUs provided better discrimination than the original set of 15 (Hunter-Gaston diversity index 0.870 vs 0.625). Loci MIRU 26, 1982, and 3232 were the most polymorphic in Beijing isolates.
Issue Date: 2-Oct-2006
URI: http://hdl.handle.net/10044/1/23785
DOI: http://dx.doi.org/10.3201/eid1209.041263
ISSN: 1080-6040
Start Page: 1406
End Page: 1413
Journal / Book Title: Emerg Infect Dis
Volume: 12
Issue: 9
Copyright Statement: Emerging Infectious Diseases is published by the Centers for Disease Control and Prevention, a U.S. Government agency. Therefore, all materials published in Emerging Infectious Diseases are in the public domain and can be used without permission. Proper citation, however, is required. Disclaimer: The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions.
Conference Place: United States
Appears in Collections:Department of Medicine
Faculty of Medicine



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