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RNA-seq: from technology to biology.

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Title: RNA-seq: from technology to biology.
Authors: Marguerat, S
Bähler, J
Item Type: Journal Article
Abstract: Next-generation sequencing technologies are now being exploited not only to analyse static genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these powerful and rapidly evolving technologies have only been available for a couple of years, they are already making substantial contributions to our understanding of genome expression and regulation. Here, we briefly describe technical issues accompanying RNA-seq data generation and analysis, highlighting differences to array-based approaches. We then review recent biological insight gained from applying RNA-seq and related approaches to deeply sample transcriptomes in different cell types or physiological conditions. These approaches are providing fascinating information about transcriptional and post-transcriptional gene regulation, and they are also giving unique insight into the richness of transcript structures and processing on a global scale and at unprecedented resolution.
Issue Date: 27-Oct-2009
URI: http://hdl.handle.net/10044/1/21975
DOI: https://dx.doi.org/10.1007/s00018-009-0180-6
ISSN: 1420-9071
Publisher: Springer Verlag
Start Page: 569
End Page: 579
Journal / Book Title: Cellular and Molecular Life Sciences
Volume: 67
Issue: 4
Copyright Statement: © The Author(s) 2009. This article is published with open access at http://link.springer.com/article/10.1007%2Fs00018-009-0180-6
Publication Status: Published
Appears in Collections:Clinical Sciences
Molecular Sciences
Faculty of Medicine



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