Hopkinson, BMBMHopkinsonDesler, CCDeslerKalisz, MMKaliszVestentoft, PSPSVestentoftJuel Rasmussen, LLJuel RasmussenBisgaard, HCHCBisgaard2017-09-042017-02-062017-09-042017-02-06Oxidative Medicine and Cellular Longevity, 2017, 20171942-0900http://hdl.handle.net/10044/1/50022Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.Copyright © 2017 Branden M. Hopkinson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Cell DifferentiationCell LineCell RespirationEnergy MetabolismFluorescent Antibody Technique, IndirectHepatocytesHuman Embryonic Stem CellsHumansMicroscopy, ConfocalMitochondriaPolymerase Chain ReactionJournal Articlehttps://www.dx.doi.org/10.1155/2017/50801281942-0994