Farahi, NedaNedaFarahiGillett, DanielDanielGillettSouthwood, MarkMarkSouthwoodRassl, DorisDorisRasslTregay, NicolaNicolaTregayHill, UtaUtaHillPreston, Stephen DenisStephen DenisPrestonLoutsios, ChrystallaChrystallaLoutsiosLok, Laurence Si ChongLaurence Si ChongLokHeard, SarahSarahHeardBuscombe, JohnJohnBuscombeRintoul, Robert CampbellRobert CampbellRintoulPeters, A MichaelA MichaelPetersSummers, CharlotteCharlotteSummersChilvers, Edwin REdwin RChilvers2020-09-092020-09-04Thorax, 2020, 75 (11), pp.1020-10230040-6376http://hdl.handle.net/10044/1/82307Neutrophils play an important role in the lung tumour microenvironment. We hypothesised that radiolabelled neutrophils coupled to single-photon emission CT (SPECT) may non-invasively quantify neutrophil uptake in tumours from patients with non-small cell lung cancer. We demonstrated increased uptake of radiolabelled neutrophils from the blood into tumours compared with non-specific uptake using radiolabelled transferrin. Moreover, indium-111-neutrophil activity in the tumour biopsies also correlated with myeloperoxidase (MPO)-positive neutrophils. Our data support the utility of imaging with In-111-labelled neutrophils and SPECT-CT to quantify neutrophil uptake in lung cancer.© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY. Published by BMJimaging/CT MRI etclung cancerneutrophil biologyJournal Articlehttps://www.dx.doi.org/10.1136/thoraxjnl-2020-214642https://thorax.bmj.com/content/75/11/10201468-3296