Sen, Sonia QSonia QSenChanchani, SachinSachinChanchaniSouthall, Tony DTony DSouthallDoe, Chris QChris QDoe2019-02-272019-02-272019-01-29eLife, 8http://hdl.handle.net/10044/1/67184<jats:p>Spatial and temporal cues are required to specify neuronal diversity, but how these cues are integrated in neural progenitors remains unknown. Drosophila progenitors (neuroblasts) are a good model: they are individually identifiable with relevant spatial and temporal transcription factors known. Here we test whether spatial/temporal factors act independently or sequentially in neuroblasts. We used Targeted-DamID to identify genomic binding sites of the Hunchback temporal factor in two neuroblasts (NB5-6 and NB7-4) that make different progeny. Hunchback targets were different in each neuroblast, ruling out the independent specification model. Moreover, each neuroblast had distinct open chromatin domains, which correlated with differential Hb-bound loci in each neuroblast. Importantly, Gsb/Pax3 spatial factor binding correlated with open chromatin in NB5-6, but not NB7-4. Our data support a model in which early-acting spatial factors establish neuroblast-specific open chromatin domains, leading to neuroblast-specific temporal factor binding and the production of different neurons in each neuroblast lineage.</jats:p>© 2019 Sen et al. This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.D. melanogasterDamIDchromatindevelopmental biologyneuroblastneurosciencespatial patterningstem celltemporal identityJournal Articlehttps://www.dx.doi.org/10.7554/elife.44036104567/Z/14/Z2050-084X