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Measuring conductance switching in single proteins using quantum tunneling

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Title: Measuring conductance switching in single proteins using quantum tunneling
Authors: Tang, L
Yi, L
Jiang, T
Ren, R
Paulose Nadappuram, B
Zhang, B
Wu, J
Liu, X
Lindsay, S
Ivanov, A
Edel, J
Item Type: Journal Article
Abstract: Interpreting the electrical signatures of single-proteins in electronic junctions has facilitated a better understanding of the intrinsic properties of proteins that are fundamental to chemical- and biological processes. Often such information is not accessible using ensemble and even single-molecule approaches. However, the fabrication of nanoscale single-protein junctions remains challenging as they often require sophisticated methods to ensure only a single protein is detected. We report on the fabrication of tunneling probes, direct measurement, and active control (switching) of single-protein conductance with an external field in solution. The probes allowed us to bridge a single streptavidin molecule to two independently addressable, biotin terminated electrodes and measure single protein tunneling response over long periods (up to 2 hours). We show that charge transport through the protein has multiple conductive pathways that depend on the magnitude of the applied bias. These findings open the door for the reliable fabrication of protein-based junctions, a better understanding of electronic properties of single protein molecules, and can enable their use in future protein-embedded bioelectronics applications.
Issue Date: 18-May-2022
Date of Acceptance: 29-Mar-2022
URI: http://hdl.handle.net/10044/1/96175
DOI: 10.1126/sciadv.abm8149
ISSN: 2375-2548
Publisher: American Association for the Advancement of Science
Start Page: 1
End Page: 8
Journal / Book Title: Science Advances
Volume: 8
Issue: 20
Copyright Statement: © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
Sponsor/Funder: Commission of the European Communities
Funder's Grant Number: 875525
Publication Status: Published
Online Publication Date: 2022-05-18
Appears in Collections:Chemistry
Faculty of Natural Sciences

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