Synthetic biology and bioelectrochemical tools for electrogenetic system engineering

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Title: Synthetic biology and bioelectrochemical tools for electrogenetic system engineering
Authors: Ledesma Amaro, R
Ouldridge, T
O'Hare, D
Team, I
Baldwin, G
Item Type: Journal Article
Abstract: Synthetic biology research and its industrial applications rely on deterministic spatiotemporal control of gene expression. Recently, electrochemical control of gene expression has been demonstrated in electrogenetic systems (redox-responsive promoters used alongside redox inducers and electrodes), allowing for the direct integration of electronics with biological processes. However, use of electrogenetic systems is limited by poor activity, tunability and standardisation. In this work we developed a strong, unidirectional, redox-responsive promoter before deriving a mutant promoter library with a spectrum of strengths. We constructed genetic circuits with these parts and demonstrated their activation by multiple classes of redox molecules. Finally, we demonstrated electrochemical activation of gene expression in aerobic conditions using a novel, modular bioelectrochemical device. These genetic and electrochemical tools facilitate the design and improve the performance of electrogenetic systems. Furthermore, the genetic design strategies used can be applied to other redox-responsive promoters to further expand the available tools for electrogenetics.
Date of Acceptance: 2-Mar-2022
URI: http://hdl.handle.net/10044/1/95797
DOI: 10.1126/sciadv.abm5091
ISSN: 2375-2548
Publisher: American Association for the Advancement of Science
Journal / Book Title: Science Advances
Volume: 8
Issue: 18
Copyright Statement: Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S.Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY)
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
The Royal Society
Biotechnology and Biological Sciences Research Council (BBSRC)
British Council (UK)
Commission of the European Communities
Commission of the European Communities
Funder's Grant Number: BB/R01602X/1
BB/T011408/1
NAF\R1\201187
BB/T013176/1
527429894
949080
101022370
Keywords: PixCell iGEM Team
Publication Status: Published
Appears in Collections:Bioengineering
Department of Infectious Diseases
Faculty of Medicine
Faculty of Natural Sciences
Faculty of Engineering



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