Polymer encapsulation of bacterial biosensors enables co-culture with mammalian cells

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Title: Polymer encapsulation of bacterial biosensors enables co-culture with mammalian cells
Authors: Moya-Ramirez, I
Kotidis, P
Marbiah, M
Kim, J
Kontoravdi, K
Polizzi, K
Item Type: Journal Article
Abstract: Coexistence of different populations of cells and isolation of tasks can provide enhanced robustness and adaptability or impart new functionalities to a culture. However, generating stable cocultures involving cells with vastly different growth rates can be challenging. To address this, we developed living analytics in a multilayer polymer shell (LAMPS), an encapsulation method that facilitates the coculture of mammalian and bacterial cells. We leverage LAMPS to preprogram a separation of tasks within the coculture: growth and therapeutic protein production by the mammalian cells and l-lactate biosensing by Escherichia coli encapsulated within LAMPS. LAMPS enable the formation of a synthetic bacterial–mammalian cell interaction that enables a living biosensor to be integrated into a biomanufacturing process. Our work serves as a proof-of-concept for further applications in bioprocessing since LAMPS combine the simplicity and flexibility of a bacterial biosensor with a viable method to prevent runaway growth that would disturb mammalian cell physiology.
Issue Date: 4-Mar-2022
Date of Acceptance: 23-Feb-2022
URI: http://hdl.handle.net/10044/1/95316
DOI: 10.1021/acssynbio.1c00577
ISSN: 2161-5063
Publisher: American Chemical Society
Journal / Book Title: ACS Synthetic Biology
Volume: 11
Issue: 3
Copyright Statement: © 2022 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Synth. Biol., after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acssynbio.1c00577
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: BB/S006206/1
EP/K038648/1
EP/T005297/1
Keywords: bacteria
biosensor
coculture
hydrogel encapsulation
l-lactate
mammalian cells
0304 Medicinal and Biomolecular Chemistry
0601 Biochemistry and Cell Biology
0903 Biomedical Engineering
Publication Status: Published online
Embargo Date: 2023-03-03
Online Publication Date: 2022-03-04
Appears in Collections:Chemical Engineering
Faculty of Engineering