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J-edited dIffusional proton nuclear magnetic resonance spectroscopic measurement of glycoprotein and supramolecular phospholipid biomarkers of inflammation in human serum.

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Title: J-edited dIffusional proton nuclear magnetic resonance spectroscopic measurement of glycoprotein and supramolecular phospholipid biomarkers of inflammation in human serum.
Authors: Nitschke, P
Lodge, S
Kimhofer, T
Masuda, R
Bong, S-H
Hall, D
Schäfer, H
Spraul, M
Pompe, N
Diercks, T
Bernardo-Seisdedos, G
Mato, JM
Millet, O
Susic, D
Henry, A
El-Omar, EM
Holmes, E
Lindon, JC
Nicholson, JK
Wist, J
Item Type: Journal Article
Abstract: Proton nuclear magnetic resonance (NMR) N-acetyl signals (Glyc) from glycoproteins and supramolecular phospholipids composite peak (SPC) from phospholipid quaternary nitrogen methyls in subcompartments of lipoprotein particles) can give important systemic metabolic information, but their absolute quantification is compromised by overlap with interfering resonances from lipoprotein lipids themselves. We present a J-Edited DIffusional (JEDI) proton NMR spectroscopic approach to selectively augment signals from the inflammatory marker peaks Glyc and SPCs in blood serum NMR spectra, which enables direct integration of peaks associated with molecules found in specific compartments. We explore a range of pulse sequences that allow editing based on peak J-modulation, translational diffusion, and T2 relaxation time and validate them for untreated blood serum samples from SARS-CoV-2 infected patients (n = 116) as well as samples from healthy controls and pregnant women with physiological inflammation and hyperlipidemia (n = 631). The data show that JEDI is an improved approach to selectively investigate inflammatory signals in serum and may have widespread diagnostic applicability to disease states associated with systemic inflammation.
Issue Date: 18-Jan-2022
Date of Acceptance: 22-Dec-2021
URI: http://hdl.handle.net/10044/1/93794
DOI: 10.1021/acs.analchem.1c04576
ISSN: 0003-2700
Publisher: American Chemical Society
Start Page: 1333
End Page: 1341
Journal / Book Title: Analytical Chemistry
Volume: 94
Issue: 2
Copyright Statement: © 2022 The Authors. Published by American Chemical Society. This work is published under CC BY 4.0 international license.
Keywords: Analytical Chemistry
0301 Analytical Chemistry
0399 Other Chemical Sciences
Publication Status: Published
Conference Place: United States
Online Publication Date: 2022-01-05
Appears in Collections:Department of Metabolism, Digestion and Reproduction
Faculty of Medicine



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