Holistic characterization of a salmonella typhimurium infection model using integrated molecular imaging.
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Title: | Holistic characterization of a salmonella typhimurium infection model using integrated molecular imaging. |
Authors: | Strittmatter, N Kanvatirth, P Inglese, P Race, AM Nilsson, A Dannhorn, A Kudo, H Goldin, RD Ling, S Wong, E Seeliger, F Serra, MP Hoffmann, S Maglennon, G Hamm, G Atkinson, J Jones, S Bunch, J Andrén, PE Takats, Z Goodwin, RJA Mastroeni, P |
Item Type: | Journal Article |
Abstract: | A more complete and holistic view on host-microbe interactions is needed to understand the physiological and cellular barriers that affect the efficacy of drug treatments and allow the discovery and development of new therapeutics. Here, we developed a multimodal imaging approach combining histopathology with mass spectrometry imaging (MSI) and same section imaging mass cytometry (IMC) to study the effects of Salmonella Typhimurium infection in the liver of a mouse model using the S. Typhimurium strains SL3261 and SL1344. This approach enables correlation of tissue morphology and specific cell phenotypes with molecular images of tissue metabolism. IMC revealed a marked increase in immune cell markers and localization in immune aggregates in infected tissues. A correlative computational method (network analysis) was deployed to find metabolic features associated with infection and revealed metabolic clusters of acetyl carnitines, as well as phosphatidylcholine and phosphatidylethanolamine plasmalogen species, which could be associated with pro-inflammatory immune cell types. By developing an IMC marker for the detection of Salmonella LPS, we were further able to identify and characterize those cell types which contained S. Typhimurium. |
Issue Date: | 1-Dec-2021 |
Date of Acceptance: | 15-Oct-2021 |
URI: | http://hdl.handle.net/10044/1/93662 |
DOI: | 10.1021/jasms.1c00240 |
ISSN: | 1044-0305 |
Publisher: | American Chemical Society |
Start Page: | 2791 |
End Page: | 2802 |
Journal / Book Title: | Journal of the American Society for Mass Spectrometry |
Volume: | 32 |
Issue: | 12 |
Copyright Statement: | t © 2021 American Society for Mass Spectrometry. Published by American Chemical Society. All rights reserved. RIGHTS & PERMISSIONS |
Keywords: | Salmonella infection imaging mass cytometry mass spectrometry imaging multimodal data integration network analysis Salmonella infection imaging mass cytometry mass spectrometry imaging multimodal data integration network analysis Analytical Chemistry 0301 Analytical Chemistry 0304 Medicinal and Biomolecular Chemistry 0306 Physical Chemistry (incl. Structural) |
Publication Status: | Published |
Conference Place: | United States |
Embargo Date: | Embargoed for 12 months after publication date |
Appears in Collections: | Department of Metabolism, Digestion and Reproduction |