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Mobile genetic elements drive antimicrobial resistance gene spread in Pasteurellaceae species

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Title: Mobile genetic elements drive antimicrobial resistance gene spread in Pasteurellaceae species
Authors: Da Silva, G
Gonçalves, O
Rosa, J
França, K
Bosse, JT
Santana, M
Langford, P
Bazzolli, D
Item Type: Journal Article
Abstract: Mobile genetic elements (MGEs) and antimicrobial resistance (AMR) drive important ecological relationships in microbial communities and pathogen-host interaction. In this study, we investigated the resistome-associated mobilome in 345 publicly available Pasteurellaceae genomes, a large family of Gram-negative bacteria including major human and animal pathogens. We generated a comprehensive dataset of the mobilome integrated into genomes, including 10,820 insertion sequences, 2,939 prophages, and 43 integrative and conjugative elements. Also, we assessed plasmid sequences of Pasteurellaceae. Our findings greatly expand the diversity of MGEs for the family, including a description of novel elements. We discovered that MGEs are comparable and dispersed across species and that they also co-occur in genomes, contributing to the family's ecology via gene transfer. In addition, we investigated the impact of these elements in the dissemination and shaping of AMR genes. A total of 55 different AMR genes were mapped to 721 locations in the dataset. MGEs are linked with 77.6% of AMR genes discovered, indicating their important involvement in the acquisition and transmission of such genes. This study provides an uncharted view of the Pasteurellaceae by demonstrating the global distribution of resistance genes linked with MGEs.
Issue Date: 6-Jan-2022
Date of Acceptance: 10-Nov-2021
URI: http://hdl.handle.net/10044/1/92895
DOI: 10.3389/fmicb.2021.773284
ISSN: 1664-302X
Publisher: Frontiers Media
Start Page: 1
End Page: 14
Journal / Book Title: Frontiers in Microbiology
Volume: 12
Copyright Statement: © 2022 da Silva, Gonçalves, Rosa, França, Bossé, Santana, Langford and Bazzolli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council
Biotechnology and Biological Sciences Research Council
Funder's Grant Number: BB/S002103/1
BB/S020543/1
BB/S019901/1
BB/S020543/1
Keywords: 0502 Environmental Science and Management
0503 Soil Sciences
0605 Microbiology
Publication Status: Published
Article Number: 773284
Online Publication Date: 2022-01-06
Appears in Collections:Department of Infectious Diseases
Faculty of Medicine



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