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C-phycoerythrin from Phormidium persicinum prevents acute kidney injury by attenuating oxidative and endoplasmic reticulum stress.

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Title: C-phycoerythrin from Phormidium persicinum prevents acute kidney injury by attenuating oxidative and endoplasmic reticulum stress.
Authors: Serrano Contreras, JI
Cano-Europa, E
Blas-Valdivia, V
Rojas Franco, P
Sfriso, AA
Garcia-Hernadez, C
Franco-Colín, M
Item Type: Journal Article
Abstract: C-phycoerythrin (C-PE) is a phycobiliprotein that prevents oxidative stress and cell damage. The aim of this study was to evaluate whether C-PE also counteracts endoplasmic reticulum (ER) stress as a mechanism contributing to its nephroprotective activity. After C-PE was purified from Phormidium persicinum by using size exclusion chromatography, it was characterized by spectrometry and fluorometry. A mouse model of HgCl2-induced acute kidney injury (AKI) was used to assess the effect of C-PE treatment (at 25, 50, or 100 mg/kg of body weight) on oxidative stress, the redox environment, and renal damage. ER stress was examined with the same model and C-PE treatment at 100 mg/kg. C-PE diminished oxidative stress and cell damage in a dose-dependent manner by impeding the decrease in expression of nephrin and podocin normally caused by mercury intoxication. It reduced ER stress by preventing the activation of the inositol-requiring enzyme-1α (IRE1α) pathway and avoiding caspase-mediated cell death, while leaving the expression of protein kinase RNA-like ER kinase (PERK) and activating transcription factor 6α (ATF6α) pathways unmodified. Hence, C-PE exhibited a nephroprotective effect on HgCl2-induced AKI by reducing oxidative stress and ER stress.
Issue Date: 20-Oct-2021
Date of Acceptance: 16-Oct-2021
URI: http://hdl.handle.net/10044/1/92728
DOI: 10.3390/md19110589
ISSN: 1660-3397
Publisher: MDPI AG
Start Page: 1
End Page: 19
Journal / Book Title: Marine Drugs
Volume: 19
Issue: 11
Copyright Statement: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Keywords: 0306 Physical Chemistry (incl. Structural)
1115 Pharmacology and Pharmaceutical Sciences
Medicinal & Biomolecular Chemistry
Publication Status: Published
Online Publication Date: 2021-10-20
Appears in Collections:Department of Metabolism, Digestion and Reproduction
Faculty of Medicine

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