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Snapshot imprinting: Rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers
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Title: | Snapshot imprinting: Rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers |
Authors: | Piletsky, S Piletska, E Poblocka, M Macip, S Jones, D Braga, M Cao, T Singh, R Spivey, A Aboagye, E Piletsky, S |
Item Type: | Journal Article |
Abstract: | Proteomic mapping of cell surfaces is an invaluable tool for drug development and clinical diagnostics. This work describes a new ‘snapshot imprinting’ method designed to obtain proteomic maps of cell surfaces, with the aim of identifying cell surface markers and epitopes for diagnostic and therapeutic applications. The analysis of two cancer cell lines, HN5 and MDA-MB-468, is described herein as a proof of concept, along with the selective targeting of three identified epitopes of epidermal growth factor receptor using molecularly imprinted polymer nanoparticles. 438 proteins were identified using this technique, with 283 considered to be transmembrane or extracellular proteins. The major advantage of the molecular imprinting approach developed here is the ability to analyse cell surface proteins without tedious fractionation, affinity separation or labelling. We believe that this system of protein analysis may provide a basic molecular diagnostics toolbox for precise, personalised treatment of cancer and other diseases. |
Issue Date: | 1-Dec-2021 |
Date of Acceptance: | 18-Sep-2021 |
URI: | http://hdl.handle.net/10044/1/92324 |
DOI: | 10.1016/j.nantod.2021.101304 |
ISSN: | 1748-0132 |
Publisher: | Elsevier |
Start Page: | 1 |
End Page: | 8 |
Journal / Book Title: | Nano Today: an international rapid reviews journal |
Volume: | 41 |
Copyright Statement: | © 2021 The Authors. Published by Elsevier Ltd. This is an open acess article under a CC-BY Attribution License (https://creativecommons.org/licenses/by/4.0/) |
Sponsor/Funder: | Imperial College Healthcare NHS Trust- BRC Funding Imperial College Healthcare NHS Trust- BRC Funding Medical Research Council |
Funder's Grant Number: | RDC04 79560 RDC04 |
Keywords: | Science & Technology Physical Sciences Technology Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Cancer Proteomics Molecularly imprinted polymers Epitopes Cells STEM-CELLS NANOPARTICLES PROTEOMICS SECRETION HEAD EGFR Nanoscience & Nanotechnology 0903 Biomedical Engineering 1007 Nanotechnology |
Publication Status: | Published |
Article Number: | ARTN 101304 |
Online Publication Date: | 2021-10-05 |
Appears in Collections: | Department of Surgery and Cancer Chemistry Faculty of Medicine Faculty of Natural Sciences |
This item is licensed under a Creative Commons License