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Snapshot imprinting: Rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers

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Title: Snapshot imprinting: Rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers
Authors: Piletsky, S
Piletska, E
Poblocka, M
Macip, S
Jones, D
Braga, M
Cao, T
Singh, R
Spivey, A
Aboagye, E
Piletsky, S
Item Type: Journal Article
Abstract: Proteomic mapping of cell surfaces is an invaluable tool for drug development and clinical diagnostics. This work describes a new ‘snapshot imprinting’ method designed to obtain proteomic maps of cell surfaces, with the aim of identifying cell surface markers and epitopes for diagnostic and therapeutic applications. The analysis of two cancer cell lines, HN5 and MDA-MB-468, is described herein as a proof of concept, along with the selective targeting of three identified epitopes of epidermal growth factor receptor using molecularly imprinted polymer nanoparticles. 438 proteins were identified using this technique, with 283 considered to be transmembrane or extracellular proteins. The major advantage of the molecular imprinting approach developed here is the ability to analyse cell surface proteins without tedious fractionation, affinity separation or labelling. We believe that this system of protein analysis may provide a basic molecular diagnostics toolbox for precise, personalised treatment of cancer and other diseases.
Issue Date: 1-Dec-2021
Date of Acceptance: 18-Sep-2021
URI: http://hdl.handle.net/10044/1/92324
DOI: 10.1016/j.nantod.2021.101304
ISSN: 1748-0132
Publisher: Elsevier
Start Page: 1
End Page: 8
Journal / Book Title: Nano Today: an international rapid reviews journal
Volume: 41
Copyright Statement: © 2021 The Authors. Published by Elsevier Ltd. This is an open acess article under a CC-BY Attribution License (https://creativecommons.org/licenses/by/4.0/)
Sponsor/Funder: Imperial College Healthcare NHS Trust- BRC Funding
Imperial College Healthcare NHS Trust- BRC Funding
Medical Research Council
Funder's Grant Number: RDC04 79560
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Science & Technology - Other Topics
Materials Science
Molecularly imprinted polymers
Nanoscience & Nanotechnology
0903 Biomedical Engineering
1007 Nanotechnology
Publication Status: Published
Article Number: ARTN 101304
Online Publication Date: 2021-10-05
Appears in Collections:Department of Surgery and Cancer
Faculty of Medicine
Faculty of Natural Sciences

This item is licensed under a Creative Commons License Creative Commons