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Discovery and characterization of two Nimrod superfamily members in Anopheles gambiae

Title: Discovery and characterization of two Nimrod superfamily members in Anopheles gambiae
Authors: Midega, J
Blight, J
Lombardo, F
Povelones, M
Kafatos, F
Christophides, GK
Item Type: Journal Article
Abstract: Anti-bacterial proteins in mosquitoes are known to play an important modulatory role on immune responses to infections with human pathogens including malaria parasites. In this study we characterized two members of the Anopheles gambiae Nimrod superfamily, namely AgNimB2 and AgEater. We confirm that current annotation of the An. gambiae genome incorrectly identifies AgNimB2 and AgEater as a single gene, AGAP009762. Through in silico and experimental approaches, it has been shown that AgNimB2 is a secreted protein that mediates phagocytosis of Staphylococcus aureus but not of Escherichia coli bacteria. We also reveal that this function does not involve a direct interaction of AgNimB2 with S. aureus. Therefore, AgNimB2 may act downstream of complement-like pathway activation, first requiring bacterial opsonization. In addition, it has been shown that AgNimB2 has an anti-Plasmodium effect. Conversely, AgEater is a membrane-bound protein that either functions redundantly or is dispensable for phagocytosis of E. coli or S. aureus. Our study provides insights into the role of members of the complex Nimrod superfamily in An. gambiae, the most important African vector of human malaria.
Issue Date: 15-Jan-2014
Date of Acceptance: 1-Jan-2014
URI: http://hdl.handle.net/10044/1/91807
DOI: 10.1179/204777213X13867543472674
ISSN: 2047-7724
Publisher: Taylor and Francis
Start Page: 463
End Page: 474
Journal / Book Title: Pathogens and Global Health
Volume: 107
Issue: 8
Copyright Statement: © W. S. Maney & Son Ltd 2013 MORE OpenChoice articles are open access and distributed under the terms of the Creative Commons Attribution Non-Commercial License 3.0
Keywords: Science & Technology
Life Sciences & Biomedicine
Public, Environmental & Occupational Health
Parasitology
Tropical Medicine
Anopheles gambiae
Innate immunity
Microbiota
Phagocytosis
Nimrod superfamily
NIM repeats
EGF-like repeats
GENOME-WIDE ANALYSIS
PHAGOCYTOSIS
MOSQUITO
PROTEIN
DROSOPHILA
SEQUENCE
COMPLEMENT
EXPRESSION
BACTERIAL
RECEPTOR
Anopheles gambiae,
EGF-like repeats
Innate immunity,
Microbiota,
NIM repeats,
Nimrod superfamily,
Phagocytosis,
Animals
Anopheles
Drosophila Proteins
Escherichia coli
Female
Immunity, Innate
Phagocytosis
Plasmodium
Receptors, Immunologic
Staphylococcus aureus
Animals
Anopheles
Plasmodium
Escherichia coli
Staphylococcus aureus
Drosophila Proteins
Receptors, Immunologic
Phagocytosis
Female
Immunity, Innate
Science & Technology
Life Sciences & Biomedicine
Public, Environmental & Occupational Health
Parasitology
Tropical Medicine
Anopheles gambiae
Innate immunity
Microbiota
Phagocytosis
Nimrod superfamily
NIM repeats
EGF-like repeats
GENOME-WIDE ANALYSIS
PHAGOCYTOSIS
MOSQUITO
PROTEIN
DROSOPHILA
SEQUENCE
COMPLEMENT
EXPRESSION
BACTERIAL
RECEPTOR
Publication Status: Published
Online Publication Date: 2014-01-15
Appears in Collections:Faculty of Natural Sciences



This item is licensed under a Creative Commons License Creative Commons