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Selective detection of Cu+ ions in live cells via fluorescence lifetime imaging microscopy.

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Title: Selective detection of Cu+ ions in live cells via fluorescence lifetime imaging microscopy.
Authors: Vilar, R
Priessner, M
Summers, PA
Lewis, B
Sastre, M
Ying, L
Kuimova, MK
Item Type: Journal Article
Abstract: Copper is an essential trace element in living organisms with its levels and localisation being carefully managed by the cellular machinery. However, if misregulated, deficiency or excess of copper ions can lead to several diseases. Therefore, it is important to have reliable methods to detect, monitor and visualise this metal in cells. Herein we report a new optical probe based on BODIPY, which shows a switch-on in its fluorescence intensity upon binding to copper(I), but not in the presence of high concentration of other physiologically relevant metal ions. More interestingly, binding to copper(I) leads to significant changes in the fluorescence lifetime of the new probe, which can be used to visualize copper(I) pools in lysosomes of live cells via fluorescence lifetime imaging microscopy (FLIM).
Issue Date: 18-Oct-2021
Date of Acceptance: 4-Aug-2021
URI: http://hdl.handle.net/10044/1/91266
DOI: 10.1002/anie.202109349
ISSN: 1433-7851
Publisher: Wiley
Start Page: 23148
End Page: 23153
Journal / Book Title: Angewandte Chemie International Edition
Volume: 60
Issue: 43
Copyright Statement: © 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: The Leverhulme Trust
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: RPG-2015-345
EP/I003983/1
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
copper(I)
Flim
fluorescence
lifetime
TCSPC
PLANT-CELLS
COPPER
PROBES
RECOGNITION
LYSOSOME
RELEASE
SENSOR
FLIM .
TCSPC
copper(I)
fluorescence
lifetime
Copper(I)
FLIM
TCSPC
fluorescence
lifetime
03 Chemical Sciences
Organic Chemistry
Publication Status: Published
Conference Place: Germany
Online Publication Date: 2021-08-11
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
National Heart and Lung Institute
Faculty of Medicine
Department of Brain Sciences
Faculty of Natural Sciences