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Virus-induced volatile organic compounds are detectable in exhaled breath during pulmonary infection.

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Title: Virus-induced volatile organic compounds are detectable in exhaled breath during pulmonary infection.
Authors: Kamal, F
Kumar, S
Edwards, MR
Veselkov, K
Belluomo, I
Kebadze, T
Romano, A
Trujillo-Torralbo, M-B
Shahridan Faiez, T
Walton, R
Ritchie, AI
Wiseman, DJ
Laponogov, I
Donaldson, G
Wedzicha, JA
Johnston, SL
Singanayagam, A
Hanna, GB
Item Type: Journal Article
Abstract: BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a condition punctuated by acute exacerbations commonly triggered by viral and/or bacterial infection. Early identification of exacerbation trigger is important to guide appropriate therapy but currently available tests are slow and imprecise. Volatile organic compounds (VOCs) can be detected in exhaled breath and have the potential to be rapid tissue-specific biomarkers of infection aetiology. METHODS: We used serial sampling within in vitro and in vivo studies to elucidate the dynamic changes that occur in VOC production during acute respiratory viral infection. Highly sensitive gas-chromatography mass spectrometry (GC-MS) techniques were used to measure VOC production from infected airway epithelial cell cultures and in exhaled breath samples of healthy subjects experimentally challenged with rhinovirus A16 and COPD subjects with naturally-occurring exacerbations. RESULTS: We identified a novel VOC signature comprising of decane and other related long chain alkane compounds that is induced during rhinovirus infection of cultured airway epithelial cells and is also increased in the exhaled breath of healthy subjects experimentally challenged with rhinovirus and of COPD patients during naturally-occurring viral exacerbations. These compounds correlated with magnitude of anti-viral immune responses, virus burden and exacerbation severity but were not induced by bacterial infection, suggesting they represent a specific virus-inducible signature. CONCLUSION: Our study highlights the potential for measurement of exhaled breath VOCs as rapid, non-invasive biomarkers of viral infection. Further studies are needed to determine whether measurement of these signatures could be used to guide more targeted therapy with antibiotic/antiviral agents for COPD exacerbations.
Issue Date: 28-Jul-2021
Date of Acceptance: 27-Jul-2021
URI: http://hdl.handle.net/10044/1/91220
DOI: 10.1164/rccm.202103-0660OC
ISSN: 1073-449X
Publisher: American Thoracic Society
Start Page: 1075
End Page: 1085
Journal / Book Title: American Journal of Respiratory and Critical Care Medicine
Volume: 204
Issue: 9
Copyright Statement: © 2021 by the American Thoracic Society
Sponsor/Funder: Wellcome Trust
Medical Research Council (MRC)
Funder's Grant Number: 215275/Z/19/Z
MR/V000098/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Critical Care Medicine
Respiratory System
General & Internal Medicine
chronic obstructive pulmonary disease
rhinovirus
volatile organic compound
viral infection
ACUTE EXACERBATIONS
RHINOVIRUS INFECTION
ANTIBIOTIC-THERAPY
INFLAMMATION
COPD
IDENTIFICATION
SPECTROMETRY
BACTERIA
PROTEIN
STRESS
chronic obstructive pulmonary disease
rhinovirus
viral infection
volatile organic compound
COPD
Virus infection
Volatile organic compound
11 Medical and Health Sciences
Respiratory System
Publication Status: Published
Conference Place: United States
Online Publication Date: 2021-07-28
Appears in Collections:Department of Surgery and Cancer
Department of Infectious Diseases
National Heart and Lung Institute
Faculty of Medicine