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Structure of the bacteriophage PhiKZ non-virion RNA polymerase

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Title: Structure of the bacteriophage PhiKZ non-virion RNA polymerase
Authors: DeYMartín Garrido, N
Orekhova, M
Lai Wan Loong, YTE
Litvinova, A
Ramlaul, K
Artamonova, T
Melnikov, AS
Serdobintsev, P
Aylett, CHS
Yakunina, M
Item Type: Journal Article
Abstract: Bacteriophage ΦKZ (PhiKZ) is the archetype of a family of massive bacterial viruses. It is considered to have therapeutic potential as its host, Pseudomonas aeruginosa, is an opportunistic, intrinsically antibiotic resistant, pathogen that kills tens of thousands worldwide each year. ΦKZ is an incredibly interesting virus, expressing many systems that the host already possesses. On infection, it forms a ‘nucleus’, erecting a barrier around its genome to exclude host endonucleases and CRISPR-Cas systems. ΦKZ infection is independent of the host transcriptional apparatus. It expresses two different multi-subunit RNA polymerases (RNAPs): the virion RNAP (vRNAP) is injected with the viral DNA during infection to transcribe early genes, including those encoding the non-virion RNAP (nvRNAP), which transcribes all further genes. ΦKZ nvRNAP is formed by four polypeptides thought to represent homologues of the eubacterial β/β′ subunits, and a fifth with unclear homology, but essential for transcription. We have resolved the structure of ΦKZ nvRNAP to better than 3.0 Å, shedding light on its assembly, homology, and the biological role of the fifth subunit: it is an embedded, integral member of the complex, the position, structural homology and biochemical role of which imply that it has evolved from an ancestral homologue to σ-factor.
Issue Date: 21-Jul-2021
Date of Acceptance: 24-Jun-2021
URI: http://hdl.handle.net/10044/1/90331
DOI: 10.1093/nar/gkab539
ISSN: 0305-1048
Publisher: Oxford University Press
Start Page: 7732
End Page: 7739
Journal / Book Title: Nucleic Acids Research
Volume: 49
Issue: 13
Copyright Statement: ©The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), whichpermits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Wellcome Trust
Funder's Grant Number: 206212/Z/17/Z
Keywords: Developmental Biology
05 Environmental Sciences
06 Biological Sciences
08 Information and Computing Sciences
Publication Status: Published
Online Publication Date: 2021-06-28
Appears in Collections:Department of Infectious Diseases
Faculty of Medicine



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