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Proteome-wide prediction of bacterial carbohydrate-binding proteins as a tool for understanding commensal and pathogen colonisation of the vaginal microbiome

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Title: Proteome-wide prediction of bacterial carbohydrate-binding proteins as a tool for understanding commensal and pathogen colonisation of the vaginal microbiome
Authors: Bonnardel, F
Haslam, SM
Dell, A
Feizi, T
Liu, Y
Tajadura-Ortega, V
Akune, Y
Sykes, L
Bennett, PR
MacIntyre, DA
Lisacek, F
Imberty, A
Item Type: Journal Article
Abstract: Bacteria use carbohydrate-binding proteins (CBPs), such as lectins and carbohydrate-binding modules (CBMs), to anchor to specific sugars on host surfaces. CBPs in the gut microbiome are well studied, but their roles in the vagina microbiome and involvement in sexually transmitted infections, cervical cancer and preterm birth are largely unknown. We established a classification system for lectins and designed Hidden Markov Model (HMM) profiles for data mining of bacterial genomes, resulting in identification of >100,000 predicted bacterial lectins available at unilectin.eu/bacteria. Genome screening of 90 isolates from 21 vaginal bacterial species shows that those associated with infection and inflammation produce a larger CBPs repertoire, thus enabling them to potentially bind a wider array of glycans in the vagina. Both the number of predicted bacterial CBPs and their specificities correlated with pathogenicity. This study provides new insights into potential mechanisms of colonisation by commensals and potential pathogens of the reproductive tract that underpin health and disease states.
Issue Date: 15-Jun-2021
Date of Acceptance: 20-May-2021
URI: http://hdl.handle.net/10044/1/89708
DOI: 10.1038/s41522-021-00220-9
ISSN: 2055-5008
Publisher: Nature Research
Start Page: 1
End Page: 10
Journal / Book Title: npj Biofilms and Microbiomes
Volume: 7
Issue: 1
Copyright Statement: © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Publication Status: Published
Open Access location: https://www.nature.com/articles/s41522-021-00220-9
Article Number: 49
Online Publication Date: 2021-06-15
Appears in Collections:Department of Metabolism, Digestion and Reproduction
Faculty of Natural Sciences

This item is licensed under a Creative Commons License Creative Commons