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Non-polar lipids as regulators of membrane properties in archaeal lipid bilayer mimics

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Title: Non-polar lipids as regulators of membrane properties in archaeal lipid bilayer mimics
Authors: Salvador-Castell, M
Brooks, NJ
Winter, R
Peters, J
Oger, PM
Item Type: Journal Article
Abstract: The modification of archaeal lipid bilayer properties by the insertion of apolar molecules in the lipid bilayer midplane has been proposed to support cell membrane adaptation to extreme environmental conditions of temperature and hydrostatic pressure. In this work, we characterize the insertion effects of the apolar polyisoprenoid squalane on the permeability and fluidity of archaeal model membrane bilayers, composed of lipid analogues. We have monitored large molecule and proton permeability and Laurdan generalized polarization from lipid vesicles as a function of temperature and hydrostatic pressure. Even at low concentration, squalane (1 mol%) is able to enhance solute permeation by increasing membrane fluidity, but at the same time, to decrease proton permeability of the lipid bilayer. The squalane physicochemical impact on membrane properties are congruent with a possible role of apolar intercalants on the adaptation of Archaea to extreme conditions. In addition, such intercalant might be used to cheaply create or modify chemically resistant liposomes (archeaosomes) for drug delivery.
Issue Date: 4-Jun-2021
Date of Acceptance: 2-Jun-2021
URI: http://hdl.handle.net/10044/1/89443
DOI: 10.3390/ijms22116087
ISSN: 1422-0067
Publisher: MDPI AG
Start Page: 6087
End Page: 6087
Journal / Book Title: International Journal of Molecular Sciences
Volume: 22
Issue: 11
Copyright Statement: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/J017566/1
Keywords: Chemical Physics
0399 Other Chemical Sciences
0604 Genetics
0699 Other Biological Sciences
Publication Status: Published
Open Access location: https://www.mdpi.com/1422-0067/22/11/6087
Online Publication Date: 2021-06-04
Appears in Collections:Chemistry
Biological and Biophysical Chemistry

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