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Bacterial indole-3-acetic acid influences soil nitrogen acquisition in barley and chickpea

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Title: Bacterial indole-3-acetic acid influences soil nitrogen acquisition in barley and chickpea
Authors: Gang, S
Sharma, S
Saraf, M
Buck, M
Schumacher, J
Item Type: Journal Article
Abstract: Farming of barley and chickpea is nitrogen (N) fertilizer dependent. Using strategies that increase the nitrogen use efficiency (NUE) and its components, nitrogen uptake efficiency (NUpE) and nitrogen utilization efficiency (NUtE) would reduce the N fertilizer application in the soil and its adverse environmental effects. We evaluated the effects of three different strains of diazotroph Klebsiella (K.p. SSN1, K.q. SGM81, and K.o. M5a1) to understand the role of biological nitrogen fixation (BNF) and bacterial indole-3-acetic acid (IAA) on NUE of the plants. A field study revealed that K.p. SSN1 results in profound increment of root surface area by eightfold and threefold compared to uninoculated (control) in barley and chickpea, respectively. We measured significant increase in the plant tissue nitrogen, chlorophyll content, protein content, nitrate reductase activity, and nitrate concentration in the inoculated plants (p ≤ 0.05). Treated barley and chickpea exhibited higher NUE and the components compared to the control plants (K.p. SSN1 ≥ K.q. SGM81> K.o. M5a1). Specifically, K.q. SGM81 treatment in barley increased NUpE by 72%, while in chickpea, K.p. SSN1 increased it by 187%. The substantial improvement in the NUpE and NUE by the auxin producers K.p. SSN1 and K.q. SGM81 compared with non-auxin producer K.o. M5a1 was accompanied by an augmented root architecture suggesting larger contribution of IAA over marginal contribution of BNF in nitrogen acquisition from the soil.
Issue Date: 16-Apr-2021
Date of Acceptance: 23-Mar-2021
URI: http://hdl.handle.net/10044/1/89066
DOI: 10.3390/plants10040780
ISSN: 2223-7747
Journal / Book Title: Plants
Volume: 10
Issue: 4
Copyright Statement: © 2021 by the authors.Licensee MDPI, Basel, Switzerland.This article is an open access articledistributed under the terms andconditions of the Creative CommonsAttribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Keywords: Science & Technology
Life Sciences & Biomedicine
Plant Sciences
nitrogen use efficiency
nitrogen uptake efficiency
nitrogen utilization efficiency
Klebsiella
indole acetic acid
root architecture
GROWTH-PROMOTING RHIZOBACTERIA
USE EFFICIENCY
REDUCTASE-ACTIVITY
TRITICUM-AESTIVUM
NITRATE
ROOT
AZOSPIRILLUM
INOCULATION
TRANSPORT
FIXATION
Klebsiella
indole acetic acid
nitrogen uptake efficiency
nitrogen use efficiency
nitrogen utilization efficiency
root architecture
Publication Status: Published
Article Number: ARTN 780
Appears in Collections:Cell and Molecular Biology
Faculty of Natural Sciences



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