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Rapid uropathogen identification using surface enhanced Raman spectroscopy active filters.

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Title: Rapid uropathogen identification using surface enhanced Raman spectroscopy active filters.
Authors: Dryden, SD
Anastasova, S
Satta, G
Thompson, AJ
Leff, DR
Darzi, A
Item Type: Journal Article
Abstract: Urinary tract infection is one of the most common bacterial infections leading to increased morbidity, mortality and societal costs. Current diagnostics exacerbate this problem due to an inability to provide timely pathogen identification. Surface enhanced Raman spectroscopy (SERS) has the potential to overcome these issues by providing immediate bacterial classification. To date, achieving accurate classification has required technically complicated processes to capture pathogens, which has precluded the integration of SERS into rapid diagnostics. This work demonstrates that gold-coated membrane filters capture and aggregate bacteria, separating them from urine, while also providing Raman signal enhancement. An optimal gold coating thickness of 50 nm was demonstrated, and the diagnostic performance of the SERS-active filters was assessed using phantom urine infection samples at clinically relevant concentrations (105 CFU/ml). Infected and uninfected (control) samples were identified with an accuracy of 91.1%. Amongst infected samples only, classification of three bacteria (Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae) was achieved at a rate of 91.6%.
Issue Date: 22-Apr-2021
Date of Acceptance: 26-Mar-2021
URI: http://hdl.handle.net/10044/1/88349
DOI: 10.1038/s41598-021-88026-9
ISSN: 2045-2322
Publisher: Nature Publishing Group
Start Page: 1
End Page: 10
Journal / Book Title: Scientific Reports
Volume: 11
Issue: 1
Copyright Statement: © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Sponsor/Funder: National Institute of Health Research
Imperial College Healthcare NHS Trust- BRC Funding
Imperial College Healthcare NHS Trust- BRC Funding
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: RDB04 79560
Publication Status: Published
Conference Place: England
Open Access location: https://www.nature.com/articles/s41598-021-88026-9
Online Publication Date: 2021-04-22
Appears in Collections:Department of Surgery and Cancer
Institute of Global Health Innovation

This item is licensed under a Creative Commons License Creative Commons