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A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules

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Title: A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules
Authors: Gutierrez-Escribano, P
Newton, MD
Llauro, A
Huber, J
Tanasie, L
Davy, J
Aly, I
Aramayo, R
Montoya, A
Kramer, H
Stigler, J
Rueda, DS
Aragon, L
Item Type: Journal Article
Abstract: Sister chromatid cohesion requires cohesin to act as a protein linker to hold chromatids together. How cohesin tethers chromatids remains poorly understood. We have used optical tweezers to visualize cohesin as it holds DNA molecules. We show that cohesin complexes tether DNAs in the presence of Scc2/Scc4 and ATP demonstrating a conserved activity from yeast to humans. Cohesin forms two classes of tethers: a “permanent bridge” resisting forces over 80 pN and a force-sensitive “reversible bridge.” The establishment of bridges requires physical proximity of dsDNA segments and occurs in a single step. “Permanent” cohesin bridges slide when they occur in trans, but cannot be removed when in cis. Therefore, DNAs occupy separate physical compartments in cohesin molecules. We finally demonstrate that cohesin tetramers can compact linear DNA molecules stretched by very low force (below 1 pN), consistent with the possibility that, like condensin, cohesin is also capable of loop extrusion.
Issue Date: 27-Nov-2019
Date of Acceptance: 22-Oct-2019
URI: http://hdl.handle.net/10044/1/88132
DOI: 10.1126/sciadv.aay6804
ISSN: 2375-2548
Publisher: American Association for the Advancement of Science
Start Page: 1
End Page: 15
Journal / Book Title: Science Advances
Volume: 5
Issue: 11
Copyright Statement: © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Wellcome Trust
Funder's Grant Number: 100955/Z/13/Z
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SISTER-CHROMATID COHESION
CHROMOSOME CONDENSATION
TRANSLOCATION
PROTEINS
REVEALS
COMPLEX
Adenosine Triphosphate
Cell Cycle Proteins
Chromatids
Chromosomal Proteins, Non-Histone
DNA, Fungal
Humans
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Chromatids
Humans
Saccharomyces cerevisiae
Cell Cycle Proteins
Saccharomyces cerevisiae Proteins
Chromosomal Proteins, Non-Histone
DNA, Fungal
Adenosine Triphosphate
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SISTER-CHROMATID COHESION
CHROMOSOME CONDENSATION
TRANSLOCATION
PROTEINS
REVEALS
COMPLEX
Publication Status: Published
Open Access location: https://advances.sciencemag.org/content/5/11/eaay6804
Article Number: ARTN eaay6804
Online Publication Date: 2019-11-27
Appears in Collections:Department of Infectious Diseases
Institute of Clinical Sciences



This item is licensed under a Creative Commons License Creative Commons