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AL-PHA beads: bioplastic-based protease biosensors for global health applications

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Title: AL-PHA beads: bioplastic-based protease biosensors for global health applications
Authors: Kelwick, RJR
Webb, AJ
Wang, Y
Heliot, A
Allan, F
Emery, AM
Templeton, MR
Freemont, PS
Item Type: Journal Article
Abstract: Proteases are multi-functional proteolytic enzymes that have complex roles in human health and disease. Therefore, the development of protease biosensors can be beneficial to global health applications. To this end, we developed Advanced proteoLytic detector PolyHydroxyAlkanoates (AL-PHA) beads – a library of over 20 low-cost, biodegradable, bioplastic-based protease biosensors. Broadly, these biosensors utilise PhaC-reporter fusion proteins that are bound to microbially manufactured polyhydroxyalkanoate beads. In the presence of a specific protease, superfolder green fluorescent reporter proteins are cleaved from the AL-PHA beads – resulting in a loss of bead fluorescence. The Tobacco Etch Virus (TEV) AL-PHA biosensor detected the proteolytic activity of at least 1.85 pM of AcTEV. AL-PHA beads were also engineered to detect cercarial elastase from Schistosoma mansoni-derived cercarial transformation fluid (SmCTF) samples, as well as cancer-associated metalloproteinases in extracellular vesicle and cell-conditioned media samples. We envision that AL-PHA beads could be further developed for use in resource-limited settings.
Issue Date: 1-Jul-2021
Date of Acceptance: 11-Feb-2021
URI: http://hdl.handle.net/10044/1/87883
DOI: 10.1016/j.mattod.2021.02.018
ISSN: 1369-7021
Publisher: Elsevier
Start Page: 25
End Page: 37
Journal / Book Title: Materials Today
Volume: 47
Copyright Statement: © 2021 The Author(s). Published by Elsevier Ltd.https://doi.org/10.1016/j.mattod.2021.02.018. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Cancer Research UK
Funder's Grant Number: EP/P028519/1
25147
Keywords: Science & Technology
Technology
Materials Science, Multidisciplinary
Materials Science
MATRIX METALLOPROTEINASES
DISINTEGRIN
BIOLOGY
Materials
03 Chemical Sciences
09 Engineering
Publication Status: Published online
Online Publication Date: 2021-03-16
Appears in Collections:Civil and Environmental Engineering
Department of Infectious Diseases
Faculty of Medicine
Faculty of Engineering



This item is licensed under a Creative Commons License Creative Commons