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Imaging non-classical mechanical responses of lipid membranes using molecular rotors

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Title: Imaging non-classical mechanical responses of lipid membranes using molecular rotors
Authors: Paez-Perez, M
Lopez-Duarte, I
Vysniauskas, A
Brooks, NJ
Kuimova, MK
Item Type: Journal Article
Abstract: Lipid packing in cellular membranes has a direct effect on membrane tension and microviscosity, and plays a central role in cellular adaptation, homeostasis and disease. According to conventional mechanical descriptions, viscosity and tension are directly interconnected, with increased tension leading to decreased membrane microviscosity. However, the intricate molecular interactions that combine to build the structure and function of a cell membrane suggest a more complex relationship between these parameters. In this work, a viscosity-sensitive fluorophore (‘molecular rotor’) is used to map changes in microviscosity in model membranes under conditions of osmotic stress. Our results suggest that the relationship between membrane tension and microviscosity is strongly influenced by the bilayer's lipid composition. In particular, we show that the effects of increasing tension are minimised for membranes that exhibit liquid disordered (Ld) – liquid ordered (Lo) phase coexistence; while, surprisingly, membranes in pure gel and Lo phases exhibit a negative compressibility behaviour, i.e. they soften upon compression.
Issue Date: 21-Feb-2021
Date of Acceptance: 22-Dec-2020
URI: http://hdl.handle.net/10044/1/87629
DOI: 10.1039/d0sc05874b
ISSN: 2041-6520
Publisher: Royal Society of Chemistry
Start Page: 2604
End Page: 2613
Journal / Book Title: Chemical Science
Volume: 12
Issue: 7
Copyright Statement: © 2021 The Author(s). Published by the Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/I003983/1
EP/J017566/1
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
03 Chemical Sciences
Publication Status: Published
Open Access location: https://doi.org/10.1039/D0SC05874B
Online Publication Date: 2020-12-22
Appears in Collections:Chemistry
Biological and Biophysical Chemistry



This item is licensed under a Creative Commons License Creative Commons