The kleboxymycin biosynthetic gene cluster is encoded by several species belonging to the Klebsiella oxytoca complex

Title: The kleboxymycin biosynthetic gene cluster is encoded by several species belonging to the Klebsiella oxytoca complex
Authors: Shibu, P
McCuaig, F
McCartney, A
Kujawska, M
Hall, L
Hoyles, L
Item Type: Working Paper
Abstract: As part of ongoing studies with clinically relevant Klebsiella spp., we characterized the genomes of three clinical GES-5-positive strains originally identified as Klebsiella oxytoca. Average nucleotide identity and phylogenetic analyses showed the strains to be Klebsiella michiganensis. In addition to encoding GES-5, the strains encoded SHV-66, a β-lactamase not previously identified in K. michiganensis. The strains further encoded a range of virulence factors and the kleboxymycin biosynthetic gene cluster (BGC), previously only found in K. oxytoca strains and one strain of Klebsiella grimontii. This BGC, associated with antibiotic-associated haemorrhagic colitis, has not previously been reported in K. michiganensis, and this finding led us to carry out a wide-ranging study to determine the prevalence of this BGC in Klebsiella spp. Of 7,170 publicly available Klebsiella genome sequences screened, 88 encoded the kleboxymycin BGC. All BGC-positive strains belonged to the K. oxytoca complex, with strains of four (K. oxytoca, K. pasteurii, K. grimontii, K. michiganensis) of the six species of the complex found to encode the complete BGC. In addition to being found in K. grimontii strains isolated from preterm infants, the BGC was found in K. oxytoca and K. michiganensis metagenome-assembled genomes recovered from neonates. Detection of the kleboxymycin BGC across the K. oxytoca complex may be of clinical relevance and this cluster should be included in databases characterizing virulence factors, in addition to those characterizing BGCs.
Issue Date: 24-Jul-2020
URI: http://hdl.handle.net/10044/1/87328
DOI: 10.1101/2020.07.24.215400
Publisher: bioRxiv
Copyright Statement: © 2020 The Author(s). It is made available under a CC-BY-NC-ND 4.0 International license.
Sponsor/Funder: Medical Research Council (MRC)
Medical Research Council (MRC)
Funder's Grant Number: MR/L01632X/1
MR/L01632X/1
Publication Status: Published
Open Access location: https://www.biorxiv.org/content/10.1101/2020.07.24.215400v1
Appears in Collections:Department of Surgery and Cancer



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