Genome characterization of a novel Wastewater bacteroides fragilis bacteriophage (vB_BfrS_23) and its host GB124

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Title: Genome characterization of a novel Wastewater bacteroides fragilis bacteriophage (vB_BfrS_23) and its host GB124
Authors: Tariq, MA
Newberry, F
Haagmans, R
Booth, C
Wileman, T
Hoyles, L
Clokie, MRJ
Ebdon, J
Carding, SR
Item Type: Journal Article
Abstract: Bacteroides spp. are part of the human intestinal microbiota but can under some circumstances become clinical pathogens. Phages are a potentially valuable therapeutic treatment option for many pathogens, but phage therapy for pathogenic Bacteroides spp. including Bacteroides fragilis is currently limited to three genome-sequenced phages. Here we describe the isolation from sewage wastewater and genome of a lytic phage, vB_BfrS_23, that infects and kills B. fragilis strain GB124. Transmission electron microscopy identified this phage as a member of the Siphoviridae family. The phage is stable when held at temperatures of 4 and 60°C for 1 h. It has a very narrow host range, only infecting one host from a panel of B. fragilis strains (n = 8). Whole-genome sequence analyses of vB_BfrS_23 determined it is double-stranded DNA phage and is circularly permuted, with a genome of 48,011 bp. The genome encodes 73 putative open reading frames. We also sequenced the host bacterium, B. fragilis GB124 (5.1 Mb), which has two plasmids of 43,923 and 4,138 bp. Although this phage is host specific, its isolation together with the detailed characterization of the host B. fragilis GB124 featured in this study represent a useful starting point from which to facilitate the future development of highly specific therapeutic agents. Furthermore, the phage could be a novel tool in determining water (and water reuse) treatment efficacy, and for identifying human fecal transmission pathways within contaminated environmental waters and foodstuffs.
Issue Date: 23-Oct-2020
Date of Acceptance: 5-Oct-2020
URI: http://hdl.handle.net/10044/1/87322
DOI: 10.3389/fmicb.2020.583378
ISSN: 1664-302X
Publisher: Frontiers Media
Start Page: 1
End Page: 12
Journal / Book Title: Frontiers in Microbiology
Volume: 11
Copyright Statement: © 2020 Tariq, Newberry, Haagmans, Booth, Wileman, Hoyles, Clokie, Ebdon and Carding. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Sponsor/Funder: Medical Research Council (MRC)
Medical Research Council (MRC)
Funder's Grant Number: MR/L01632X/1
MR/L01632X/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Microbiology
bacteriophage
Bacteroides fragilis
genomes
waste water
human
ANTIMICROBIAL RESISTANCE
FECAL POLLUTION
VIRAL PATHOGENS
SAMPLES
PROTEIN
VERSION
GB-124
INDICATORS
SECRETION
MECHANISM
Bacteroides fragilis
bacteriophage
genomes
human
waste water
Science & Technology
Life Sciences & Biomedicine
Microbiology
bacteriophage
Bacteroides fragilis
genomes
waste water
human
ANTIMICROBIAL RESISTANCE
FECAL POLLUTION
VIRAL PATHOGENS
SAMPLES
PROTEIN
VERSION
GB-124
INDICATORS
SECRETION
MECHANISM
0502 Environmental Science and Management
0503 Soil Sciences
0605 Microbiology
Publication Status: Published
Open Access location: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644841/
Article Number: ARTN 583378
Online Publication Date: 2020-10-23
Appears in Collections:Department of Surgery and Cancer



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