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Hand-portable HPLC with broadband spectral detection enables analysis of complex polycyclic aromatic hydrocarbon mixtures

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Title: Hand-portable HPLC with broadband spectral detection enables analysis of complex polycyclic aromatic hydrocarbon mixtures
Authors: Chatzimichail, S
Rahimi, F
Saifuddin, A
Surman, A
Taylor-Robinson, S
Salehi-Reyhani, SA
Item Type: Journal Article
Abstract: Polycyclic aromatic hydrocarbons (PAHs) are considered priority hazardous substances due to their carcinogenic activity and risk to public health. Strict regulations are in place limiting their release into the environment, but enforcement is hampered by a lack of adequate field-testing procedure, instead relying on sending samples to centralised analytical facilities. Reliably monitoring levels of PAHs in the field is a challenge, owing to the lack of field-deployable analytical methods able to separate, identify, and quantify the complex mixtures in which PAHs are typically observed. Here, we report the development of a hand-portable system based on high-performance liquid chromatography incorporating a spectrally wide absorption detector, capable of fingerprinting PAHs based on their characteristic spectral absorption profiles: identifying 100% of the 24 PAHs tested, including full coverage of the United States Environmental Protection Agency priority pollutant list. We report unsupervised methods to exploit these new capabilities for feature detection and identification, robust enough to detect and classify co-eluting and hidden peaks. Identification is fully independent of their characteristic retention times, mitigating matrix effects which can preclude reliable determination of these analytes in challenging samples. We anticipate the platform to enable more sophisticated analytical measurements, supporting real-time decision making in the field.
Issue Date: 16-Feb-2021
Date of Acceptance: 19-Jan-2021
URI: http://hdl.handle.net/10044/1/87038
DOI: 10.1038/s42004-021-00457-7
ISSN: 2399-3669
Publisher: Nature Research
Journal / Book Title: Communications Chemistry
Volume: 4
Copyright Statement: © The Author(s) 2021. This article is licensed under a Creative CommonsAttribution 4.0 International License, which permits use, sharing,adaptation, distribution and reproduction in any medium or format, as long as you giveappropriate credit to the original author(s) and the source, provide a link to the CreativeCommons license, and indicate if changes were made. The images or other third partymaterial in this article are included in the article’s Creative Commons license, unlessindicated otherwise in a credit line to the material. If material is not included in thearticle’s Creative Commons license and your intended use is not permitted by statutoryregulation or exceeds the permitted use, you will need to obtain permission directly fromthe copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Sponsor/Funder: Analytical Chemistry Trust Fund
Engineering and Physical Sciences Research Council
Engineering & Physical Science Research Council (E
Funder's Grant Number: 600322/02
EP/S001603/3
EP/P510798/1
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
LIQUID-CHROMATOGRAPHY
MASS-SPECTROMETER
PAHS
PERFORMANCE
EXTRACTION
HEALTH
ENVIRONMENT
EXPOSURE
RISK
FOOD
portable chromatography
environmental chemistry
polycyclic aromatic hydrocarbons
spectral fingerprinting
chemometrics
Publication Status: Published
Article Number: ARTN 17
Appears in Collections:Department of Surgery and Cancer
Electrical and Electronic Engineering
Faculty of Medicine



This item is licensed under a Creative Commons License Creative Commons