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Innate immune responses of Galleria mellonella to Mycobacterium bovis BCG challenge identified using proteomic and molecular approaches

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Title: Innate immune responses of Galleria mellonella to Mycobacterium bovis BCG challenge identified using proteomic and molecular approaches
Authors: Asai, M
Sheehan, G
Li, Y
Robertson, B
Kavanagh, K
Langford, P
Newton, S
Item Type: Journal Article
Abstract: The larvae of the insect Galleria mellonella, have recently been established as a non-mammalian infection model for the Mycobacterium tuberculosis complex (MTBC). To gain further insight into the potential of this model, we applied proteomic (label-free quantification) and transcriptomic (gene expression) approaches to characterise the innate immune response of G. mellonella to infection with Mycobacterium bovis BCG lux over a 168 h time course. Proteomic analysis of the haemolymph from infected larvae revealed distinct changes in the proteome at all time points (4, 48, 168 h). Reverse transcriptase quantitative PCR confirmed induction of five genes (gloverin, cecropin, IMPI, hemolin, and Hdd11), which encoded proteins found to be differentially abundant from the proteomic analysis. However, the trend between gene expression and protein abundance were largely inconsistent (20%). Overall, the data are in agreement with previous phenotypic observations such as haemocyte internalization of mycobacterial bacilli (hemolin/β-actin), formation of granuloma-like structures (Hdd11), and melanization (phenoloxidase activating enzyme 3 and serpins). Furthermore, similarities in immune expression in G. mellonella, mouse, zebrafish and in vitro cell-line models of tuberculosis infection were also identified for the mechanism of phagocytosis (β-actin). Cecropins (antimicrobial peptides), which share the same α-helical motif as a highly potent peptide expressed in humans (h-CAP-18), were induced in G. mellonella in response to infection, giving insight into a potential starting point for novel antimycobacterial agents. We believe that these novel insights into the innate immune response further contribute to the validation of this cost-effective and ethically acceptable insect model to study members of the MTBC.
Issue Date: 9-Feb-2021
Date of Acceptance: 4-Jan-2021
URI: http://hdl.handle.net/10044/1/86513
DOI: 10.3389/fcimb.2021.619981
ISSN: 2235-2988
Publisher: Frontiers Media
Journal / Book Title: Frontiers in Cellular and Infection Microbiology
Volume: 11
Copyright Statement: © 2021 Asai, Sheehan, Li, Robertson, Kavanagh, Langford and Newton. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Sponsor/Funder: NC3Rs (National Centre for the Replacement, Refinement and Reduction of Animals in Research)
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: NC/R001596/1
Keywords: Galleria mellonella
Mycobacterium bovis BCG
gene expression
in vivo model
innate immunity
0601 Biochemistry and Cell Biology
0605 Microbiology
Publication Status: Published
Article Number: ARTN 619981
Appears in Collections:Department of Infectious Diseases
Faculty of Medicine

This item is licensed under a Creative Commons License Creative Commons