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Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry

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Title: Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry
Authors: Strutt, R
Hindley, JW
Gregg, J
Booth, PJ
Harling, JD
Law, RV
Friddin, MS
Ces, O
Item Type: Journal Article
Abstract: Droplet microcompartments linked by lipid bilayers show great promise in the construction of synthetic minimal tissues. Central to controlling the flow of information in these systems are membrane proteins, which can gate in response to specific stimuli in order to control the molecular flux between membrane separated compartments. This has been demonstrated with droplet interface bilayers (DIBs) using several different membrane proteins combined with electrical, mechanical, and/or chemical activators. Here we report the activation of the bacterial mechanosensitive channel of large conductance (MscL) in a dioleoylphosphatidylcholine:dioleoylphosphatidylglycerol DIB by controlling membrane asymmetry. We show using electrical measurements that the incorporation of lysophosphatidylcholine (LPC) into one of the bilayer leaflets triggers MscL gating in a concentration-dependent manner, with partial and full activation observed at 10 and 15 mol% LPC respectively. Our findings could inspire the design of new minimal tissues where flux pathways are dynamically defined by lipid composition.
Issue Date: 14-Feb-2021
Date of Acceptance: 3-Dec-2020
URI: http://hdl.handle.net/10044/1/85183
DOI: 10.1039/d0sc03889j
ISSN: 2041-6520
Publisher: Royal Society of Chemistry (RSC)
Start Page: 2138
End Page: 2145
Journal / Book Title: Chemical Science
Volume: 12
Issue: 6
Copyright Statement: © The Royal Society of Chemistry 2020. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Sponsor/Funder: Engineering & Physical Science Research Council (E
Funder's Grant Number: CHBBC_P81995
Keywords: 03 Chemical Sciences
Publication Status: Published
Open Access location: https://pubs.rsc.org/en/content/articlelanding/2021/sc/d0sc03889j#!divAbstract
Online Publication Date: 2021-01-04
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
Dyson School of Design Engineering
Faculty of Natural Sciences
Faculty of Engineering



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