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Size exclusion chromatography as a technique for the investigation of novel extracellular vesicles in cancer

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Title: Size exclusion chromatography as a technique for the investigation of novel extracellular vesicles in cancer
Authors: Liu, D
Upton, F
Rees, E
Limb, C
Jiao, L
Krell, J
Frampton, A
Item Type: Journal Article
Abstract: Abstract: (1) Background: Cancer cells release extracellular vesicles that are a rich target for biomarker discovery and provide a promising mechanism for liquid biopsy. SEC is an increasingly popular technique which has been rediscovered for the purposes of EV isolation and purification from diverse biofluids. (2) Methods: A review was undertaken to identify all papers which described size exclusion as their primary EV isolation method in cancer research. (3) Results: 37 papers were identified and discussed which showcases the breadth of applications that EVs can be utilised, from proteomics, to RNA, and through to functionality. A range of different methods are highlighted, with Sepharose-based techniques predominating. (4) Conclusions: EVs isolated using SEC are able to identify cancer cells, highlight active pathways in tumourigenesis, clinically distinguish cohorts and remain functionally active for further experiments.
Issue Date: 27-Oct-2020
Date of Acceptance: 15-Oct-2020
URI: http://hdl.handle.net/10044/1/84830
DOI: 10.3390/cancers12113156
ISSN: 2072-6694
Publisher: MDPI AG
Journal / Book Title: Cancers
Volume: 12
Issue: 11
Copyright Statement: ©2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open accessarticle distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (http://creativecommons.org/licenses/by/4.0/)
Sponsor/Funder: Royal College Of Surgeons Of England
Royal College of Surgeons Edinburgh
J P Moulton Charitable Foundation
Mason Medical Research Foundation
Funder's Grant Number: WSCC_P83671
SPPG/17/107
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Keywords: SEC
biomarkers
cancer
exosomes
extracellular vesicles
size-exclusion chromatography
1112 Oncology and Carcinogenesis
Publication Status: Published
Article Number: ARTN 3156
Appears in Collections:Department of Surgery and Cancer



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