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Hijacking the hijackers: escherichia coli pathogenicity islands redirect helper phage packaging for their own benefit

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Title: Hijacking the hijackers: escherichia coli pathogenicity islands redirect helper phage packaging for their own benefit
Authors: Fillol-Salom, A
Bacarizo, J
Alqasmi, M
Rafael Ciges-Tomas, J
Martinez-Rubio, R
Roszak, AW
Cogdell, RJ
Chen, J
Marina, A
Penades, JR
Item Type: Journal Article
Abstract: Phage-inducible chromosomal islands (PICIs) represent a novel and universal class of mobile genetic elements, which have broad impact on bacterial virulence. In spite of their relevance, how the Gram-negative PICIs hijack the phage machinery for their own specific packaging and how they block phage reproduction remains to be determined. Using genetic and structural analyses, we solve the mystery here by showing that the Gram-negative PICIs encode a protein that simultaneously performs these processes. This protein, which we have named Rpp (for redirecting phage packaging), interacts with the phage terminase small subunit, forming a heterocomplex. This complex is unable to recognize the phage DNA, blocking phage packaging, but specifically binds to the PICI genome, promoting PICI packaging. Our studies reveal the mechanism of action that allows PICI dissemination in nature, introducing a new paradigm in the understanding of the biology of pathogenicity islands and therefore of bacterial pathogen evolution.
Issue Date: 5-Sep-2019
Date of Acceptance: 13-Jun-2019
URI: http://hdl.handle.net/10044/1/84172
DOI: 10.1016/j.molcel.2019.06.017
ISSN: 1097-2765
Publisher: Cell Press
Start Page: 1020
End Page: 1030.e4
Journal / Book Title: Molecular Cell
Volume: 75
Issue: 5
Copyright Statement: © 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
BACTERIOPHAGE-LAMBDA
DNA
SYSTEM
TERMINASE
GENETICS
DOMAINS
BINDING
SERVER
GENES
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
BACTERIOPHAGE-LAMBDA
DNA
SYSTEM
TERMINASE
GENETICS
DOMAINS
BINDING
SERVER
GENES
PICI
TerS
bacteriophage
evolution
gene transfer
pathogenicity islands
pirating
transduction
virulence
Bacteriophages
DNA, Viral
Endodeoxyribonucleases
Escherichia coli
Escherichia coli Proteins
Genomic Islands
Viral Proteins
Virus Assembly
Escherichia coli
Bacteriophages
Endodeoxyribonucleases
Escherichia coli Proteins
Viral Proteins
DNA, Viral
Virus Assembly
Genomic Islands
Developmental Biology
06 Biological Sciences
11 Medical and Health Sciences
Publication Status: Published
Online Publication Date: 2019-07-23
Appears in Collections:Department of Infectious Diseases
Faculty of Medicine



This item is licensed under a Creative Commons License Creative Commons