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Dual-view oblique plane microscopy (dOPM)

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Title: Dual-view oblique plane microscopy (dOPM)
Authors: Sparks, H
Dent, L
Bakal, C
Behrens, A
Salbreux, G
Dunsby, C
Item Type: Journal Article
Abstract: We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35±0.04 μm and 0.39±0.02 μm laterally and 0.81±0.07 μm axially. The measured z-sectioning value was 1.33±0.45 μm using light-sheet FWHM in the frames of the two views of 4.99±0.58 μm and 4.89±0.63 μm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 μm in diameter was imaged.
Date of Acceptance: 29-Oct-2020
URI: http://hdl.handle.net/10044/1/83985
DOI: 10.1364/boe.409781
ISSN: 2156-7085
Publisher: The Optical Society
Journal / Book Title: Biomedical Optics Express
Copyright Statement: © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement (https://doi.org/10.1364/OA_License_v1).
Sponsor/Funder: Engineering & Physical Science Research Council (E
Funder's Grant Number: 10976 (EP/T003103/1)
Keywords: 0205 Optical Physics
0912 Materials Engineering
Publication Status: Accepted
Embargo Date: publication subject to indefinite embargo
Appears in Collections:Physics
Photonics