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In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

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Title: In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy
Authors: Hogset, H
Horgan, C
Bergholt, M
Armstrong, J
Torraca, V
Chen, Q
Keane, TJ
Bugeon, L
Dallman, M
Mostowy, S
Stevens, M
Item Type: Journal Article
Abstract: Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivotemporal monitoring of the wound response in living zebrafish embryos.Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis in fully intact and living zebrafish embryos.
Issue Date: 2-Dec-2020
Date of Acceptance: 28-Oct-2020
URI: http://hdl.handle.net/10044/1/83909
DOI: 10.1038/s41467-020-19827-1
ISSN: 2041-1723
Publisher: Nature Research
Start Page: 1
End Page: 12
Journal / Book Title: Nature Communications
Volume: 11
Copyright Statement: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Sponsor/Funder: Commission of the European Communities
Commission of the European Communities
Medical Research Council (MRC)
Commission of the European Communities
Commission of the European Communities
Wellcome Trust
Arthritis Research UK
Commission of the European Communities
Funder's Grant Number: 676137
701713
MR/S00551X/1
ERC-2013-CoG-616417
746980
098411/Z/12/Z
21138
700088
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
GENERATION MICROSCOPY
CANCER
DYNAMICS
CELLS
IDENTIFICATION
INFECTION
GROWTH
Animals
Animals, Genetically Modified
Embryo, Nonmammalian
Molecular Imaging
Multivariate Analysis
Mycobacterium Infections, Nontuberculous
Mycobacterium marinum
Spectrum Analysis, Raman
Time-Lapse Imaging
Wound Healing
Zebrafish
Embryo, Nonmammalian
Animals
Animals, Genetically Modified
Zebrafish
Mycobacterium marinum
Spectrum Analysis, Raman
Multivariate Analysis
Wound Healing
Molecular Imaging
Time-Lapse Imaging
Mycobacterium Infections, Nontuberculous
Publication Status: Published
Article Number: 6172
Online Publication Date: 2020-12-02
Appears in Collections:Materials
Faculty of Natural Sciences
Faculty of Engineering



This item is licensed under a Creative Commons License Creative Commons