42
IRUS TotalDownloads
Altmetric
In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy
| File | Description | Size | Format | |
|---|---|---|---|---|
 s41467-020-19827-1.pdf | Published version | 5.24 MB | Adobe PDF | View/Open |
| Title: | In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy |
| Authors: | Hogset, H Horgan, C Bergholt, M Armstrong, J Torraca, V Chen, Q Keane, TJ Bugeon, L Dallman, M Mostowy, S Stevens, M |
| Item Type: | Journal Article |
| Abstract: | Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivotemporal monitoring of the wound response in living zebrafish embryos.Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis in fully intact and living zebrafish embryos. |
| Issue Date: | 2-Dec-2020 |
| Date of Acceptance: | 28-Oct-2020 |
| URI: | http://hdl.handle.net/10044/1/83909 |
| DOI: | 10.1038/s41467-020-19827-1 |
| ISSN: | 2041-1723 |
| Publisher: | Nature Research |
| Start Page: | 1 |
| End Page: | 12 |
| Journal / Book Title: | Nature Communications |
| Volume: | 11 |
| Copyright Statement: | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| Sponsor/Funder: | Commission of the European Communities Commission of the European Communities Medical Research Council (MRC) Commission of the European Communities Commission of the European Communities Wellcome Trust Arthritis Research UK Commission of the European Communities |
| Funder's Grant Number: | 676137 701713 MR/S00551X/1 ERC-2013-CoG-616417 746980 098411/Z/12/Z 21138 700088 |
| Keywords: | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics GENERATION MICROSCOPY CANCER DYNAMICS CELLS IDENTIFICATION INFECTION GROWTH Animals Animals, Genetically Modified Embryo, Nonmammalian Molecular Imaging Multivariate Analysis Mycobacterium Infections, Nontuberculous Mycobacterium marinum Spectrum Analysis, Raman Time-Lapse Imaging Wound Healing Zebrafish Embryo, Nonmammalian Animals Animals, Genetically Modified Zebrafish Mycobacterium marinum Spectrum Analysis, Raman Multivariate Analysis Wound Healing Molecular Imaging Time-Lapse Imaging Mycobacterium Infections, Nontuberculous |
| Publication Status: | Published |
| Article Number: | 6172 |
| Online Publication Date: | 2020-12-02 |
| Appears in Collections: | Materials Faculty of Natural Sciences Faculty of Engineering |
This item is licensed under a Creative Commons License
