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Fast, easy and early (larval) identification of transparent mutant zebrafish using standard fluorescence microscopy [version 1; peer review: 2 approved]

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Title: Fast, easy and early (larval) identification of transparent mutant zebrafish using standard fluorescence microscopy [version 1; peer review: 2 approved]
Authors: Wenz, R
Conibear, E
Bugeon, L
Dallman, M
Item Type: Journal Article
Abstract: The availability of transparent zebrafish mutants (either TraNac: tra b6/b6; nac w2/w2 or casper: roy a9/a9; nac w2/w2 ) for live imaging studies together with the ease of generating transgenic lines are two of the strengths of the zebrafish model organism. The fact that transparent casper ( roy a9/a9;nac w2/w2) and silver nacre ( nac w2/w2) mutants are indistinguishable by eye at early stages (1-5 days post-fertilization; dpf) means many fish must be raised and later culled if they are not transparent. To identify translucent mutants early and easily at the early larval stage (≤5 dpf) before they are classified as protected animals, we developed a simple screening method using standard fluorescence microscopy. We estimate that this procedure could annually save 60,000 animals worldwide.
Issue Date: 10-Aug-2020
Date of Acceptance: 9-Jul-2020
URI: http://hdl.handle.net/10044/1/83118
DOI: 10.12688/f1000research.22464.1
ISSN: 2046-1402
Publisher: F1000 Research Ltd
Journal / Book Title: F1000Research
Volume: 9
Copyright Statement: © 2020 Wenz R et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Zebrafish
casper
iridophore
nac
screening
tra
trab6/b6nacw2/w2
translucent
transparent
Zebrafish
casper
iridophore
nac
screening
tra
trab6/b6nacw2/w2
translucent
transparent
0601 Biochemistry and Cell Biology
1103 Clinical Sciences
1112 Oncology and Carcinogenesis
Publication Status: Published online
Conference Place: England
Article Number: ARTN 963
Appears in Collections:Faculty of Natural Sciences



This item is licensed under a Creative Commons License Creative Commons