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Site-specific characterisation of SARS-CoV-2 spike glycoprotein receptor binding domain

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Title: Site-specific characterisation of SARS-CoV-2 spike glycoprotein receptor binding domain
Authors: Antonopoulos, A
Broome, S
Sharov, V
Ziegenfuss, C
Easton, RL
Panico, M
Morris, HR
Haslam, S
Item Type: Journal Article
Abstract: The novel coronavirus SARS-CoV-2, the infective agent causing COVID-19, is having a global impact both in terms of human disease as well as socially and economically. Its heavily glycosylated spike glycoprotein is fundamental for the infection process, via its receptor binding domains interaction with the glycoprotein angiotensin converting enzyme 2 on human cell surfaces. We therefore utilized an integrated glycomic and glycoproteomic analytical strategy to characterise both N- and O- glycan site specific glycosylation within the receptor binding domain. We demonstrate the presence of complex type N-glycans with unusual fucosylated LacdiNAc at both sites N331 and N343 and a single site of O-glycosylation on T323.
Issue Date: 1-Mar-2021
Date of Acceptance: 24-Aug-2020
URI: http://hdl.handle.net/10044/1/82154
DOI: 10.1093/glycob/cwaa085
ISSN: 0959-6658
Publisher: Oxford University Press (OUP)
Start Page: 181
End Page: 187
Journal / Book Title: Glycobiology
Volume: 31
Issue: 3
Copyright Statement: © The Author(s) 2020. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited
Keywords: SARS-CoV-2
glycoproteomics
mass spectrometry
spike glycoprotein
Angiotensin-Converting Enzyme 2
Binding Sites
COVID-19
Carbohydrate Conformation
Carbohydrate Sequence
Glycomics
Glycosylation
HEK293 Cells
Host Microbial Interactions
Humans
Pandemics
Protein Binding
Protein Interaction Domains and Motifs
Proteomics
Receptors, Virus
Recombinant Proteins
SARS-CoV-2
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spike Glycoprotein, Coronavirus
Biochemistry & Molecular Biology
06 Biological Sciences
11 Medical and Health Sciences
Publication Status: Published
Online Publication Date: 2020-09-03
Appears in Collections:Imperial College London COVID-19
Faculty of Natural Sciences



This item is licensed under a Creative Commons License Creative Commons