6
IRUS TotalDownloads
Altmetric
Site-specific characterisation of SARS-CoV-2 spike glycoprotein receptor binding domain
File | Description | Size | Format | |
---|---|---|---|---|
![]() | Published version | 2.03 MB | Adobe PDF | View/Open |
Title: | Site-specific characterisation of SARS-CoV-2 spike glycoprotein receptor binding domain |
Authors: | Antonopoulos, A Broome, S Sharov, V Ziegenfuss, C Easton, RL Panico, M Morris, HR Haslam, S |
Item Type: | Journal Article |
Abstract: | The novel coronavirus SARS-CoV-2, the infective agent causing COVID-19, is having a global impact both in terms of human disease as well as socially and economically. Its heavily glycosylated spike glycoprotein is fundamental for the infection process, via its receptor binding domains interaction with the glycoprotein angiotensin converting enzyme 2 on human cell surfaces. We therefore utilized an integrated glycomic and glycoproteomic analytical strategy to characterise both N- and O- glycan site specific glycosylation within the receptor binding domain. We demonstrate the presence of complex type N-glycans with unusual fucosylated LacdiNAc at both sites N331 and N343 and a single site of O-glycosylation on T323. |
Issue Date: | 1-Mar-2021 |
Date of Acceptance: | 24-Aug-2020 |
URI: | http://hdl.handle.net/10044/1/82154 |
DOI: | 10.1093/glycob/cwaa085 |
ISSN: | 0959-6658 |
Publisher: | Oxford University Press (OUP) |
Start Page: | 181 |
End Page: | 187 |
Journal / Book Title: | Glycobiology |
Volume: | 31 |
Issue: | 3 |
Copyright Statement: | © The Author(s) 2020. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited |
Keywords: | SARS-CoV-2 glycoproteomics mass spectrometry spike glycoprotein Angiotensin-Converting Enzyme 2 Binding Sites COVID-19 Carbohydrate Conformation Carbohydrate Sequence Glycomics Glycosylation HEK293 Cells Host Microbial Interactions Humans Pandemics Protein Binding Protein Interaction Domains and Motifs Proteomics Receptors, Virus Recombinant Proteins SARS-CoV-2 Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spike Glycoprotein, Coronavirus Biochemistry & Molecular Biology 06 Biological Sciences 11 Medical and Health Sciences |
Publication Status: | Published |
Online Publication Date: | 2020-09-03 |
Appears in Collections: | Imperial College London COVID-19 Faculty of Natural Sciences |
This item is licensed under a Creative Commons License