Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail

Title: Brainstem respiratory oscillators develop independently of neuronal migration defects in the Wnt/PCP mouse mutant looptail
Authors: Thoby-Brisson, M
Bouvier, J
Glasco, DM
Stewart, ME
Dean, C
Murdoch, JN
Champagnat, J
Fortin, G
Chandrasekhar, A
Item Type: Journal Article
Abstract: The proper development and maturation of neuronal circuits require precise migration of component neurons from their birthplace (germinal zone) to their final positions. Little is known about the effects of aberrant neuronal position on the functioning of organized neuronal groups, especially in mammals. Here, we investigated the formation and properties of brainstem respiratory neurons in looptail (Lp) mutant mice in which facial motor neurons closely apposed to some respiratory neurons fail to migrate due to loss of function of the Wnt/Planar Cell Polarity (PCP) protein Vangl2. Using calcium imaging and immunostaining on embryonic hindbrain preparations, we found that respiratory neurons constituting the embryonic parafacial oscillator (e-pF) settled at the ventral surface of the medulla in Vangl2Lp/+ and Vangl2Lp/Lp embryos despite the failure of tangential migration of its normally adjacent facial motor nucleus. Anatomically, the e-pF neurons were displaced medially in Lp/+ embryos and rostro-medially Lp/Lp embryos. Pharmacological treatments showed that the e-pF oscillator exhibited characteristic network properties in both Lp/+ and Lp/Lp embryos. Furthermore, using hindbrain slices, we found that the other respiratory oscillator, the preBötzinger complex, was also anatomically and functionally established in Lp mutants. Importantly, the displaced e-pF oscillator established functional connections with the preBötC oscillator in Lp/+ mutants. Our data highlight the robustness of the developmental processes that assemble the neuronal networks mediating an essential physiological function.
Issue Date: 17-Feb-2012
Date of Acceptance: 3-Jan-2012
URI: http://hdl.handle.net/10044/1/81445
DOI: 10.1371/journal.pone.0031140
ISSN: 1932-6203
Publisher: Public Library of Science (PLoS)
Start Page: 1
End Page: 12
Journal / Book Title: PLoS One
Volume: 7
Issue: 2
Copyright Statement: © 2012 Thoby-Brisson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
BRANCHIOMOTOR NEURONS
MOTOR-NEURONS
NEURAL-TUBE
HINDBRAIN
PHOX2B
RHYTHM
GENE
CHEMOSENSITIVITY
ZEBRAFISH
IDENTIFICATION
Animals
Biological Clocks
Brain Stem
Cell Movement
Cell Polarity
Embryo, Mammalian
Face
Female
Homeodomain Proteins
Hydrogen-Ion Concentration
LIM-Homeodomain Proteins
Mice
Mice, Mutant Strains
Models, Biological
Motor Neurons
Neurons
Respiration
Rhombencephalon
Transcription Factors
Wnt Proteins
Face
Brain Stem
Rhombencephalon
Neurons
Motor Neurons
Animals
Mice
Mice, Mutant Strains
Homeodomain Proteins
Transcription Factors
Cell Movement
Cell Polarity
Biological Clocks
Respiration
Hydrogen-Ion Concentration
Models, Biological
Female
Wnt Proteins
Embryo, Mammalian
LIM-Homeodomain Proteins
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
BRANCHIOMOTOR NEURONS
MOTOR-NEURONS
NEURAL-TUBE
HINDBRAIN
PHOX2B
RHYTHM
GENE
CHEMOSENSITIVITY
ZEBRAFISH
IDENTIFICATION
General Science & Technology
Publication Status: Published
Article Number: ARTN e31140
Online Publication Date: 2012-02-17
Appears in Collections:National Heart and Lung Institute



This item is licensed under a Creative Commons License Creative Commons