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Cell-surface receptors enable perception of extracellular cytokinins

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Title: Cell-surface receptors enable perception of extracellular cytokinins
Authors: Antoniadi, I
Novák, O
Gelová, Z
Johnson, A
Plíhal, O
Simerský, R
Mik, V
Vain, T
Mateo-Bonmatí, E
Karady, M
Pernisová, M
Plačková, L
Opassathian, K
Hejátko, J
Robert, S
Friml, J
Doležal, K
Ljung, K
Turnbull, C
Item Type: Journal Article
Abstract: Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.
Issue Date: 27-Aug-2020
Date of Acceptance: 14-Jul-2020
URI: http://hdl.handle.net/10044/1/81416
DOI: 10.1038/s41467-020-17700-9
ISSN: 2041-1723
Publisher: Nature Research
Start Page: 1
End Page: 10
Journal / Book Title: Nature Communications
Volume: 11
Copyright Statement: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
Publication Status: Published
Online Publication Date: 2020-08-27
Appears in Collections:Faculty of Natural Sciences

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