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Comparative primary paediatric nasal epithelial cell culture differentiation and RSV-induced cytopathogenesis following culture in two commercial media

Title: Comparative primary paediatric nasal epithelial cell culture differentiation and RSV-induced cytopathogenesis following culture in two commercial media
Authors: Broadbent, L
Manzoor, S
Zarcone, MC
Barabas, J
Shields, MD
Saglani, S
Lloyd, CM
Bush, A
Custovic, A
Ghazal, P
Gore, M
Marsland, B
Roberts, G
Schwarze, J
Turner, S
Power, UF
Item Type: Journal Article
Abstract: The culture of differentiated human airway epithelial cells allows the study of pathogen-host interactions and innate immune responses in a physiologically relevant in vitro model. As the use of primary cell culture has gained popularity the availability of the reagents needed to generate these cultures has increased. In this study we assessed two different media, Promocell and PneumaCult, during the differentiation and maintenance of well-differentiated primary nasal epithelial cell cultures (WD-PNECs). We compared and contrasted the consequences of these media on WD-PNEC morphological and physiological characteristics and their responses to respiratory syncytial virus (RSV) infection. We found that cultures generated using PneumaCult resulted in greater total numbers of smaller, tightly packed, pseudostratified cells. However, cultures from both media resulted in similar proportions of ciliated and goblet cells. There were no differences in RSV growth kinetics, although more ciliated cells were infected in the PneumaCult cultures. There was also significantly more IL-29/IFNλ1 secreted from PneumaCult compared to Promocell cultures following infection. In conclusion, the type of medium used for the differentiation of primary human airway epithelial cells may impact experimental results.
Issue Date: 26-Mar-2020
Date of Acceptance: 5-Mar-2020
URI: http://hdl.handle.net/10044/1/81157
DOI: 10.1371/journal.pone.0228229
ISSN: 1932-6203
Publisher: Public Library of Science (PLoS)
Start Page: 1
End Page: 12
Journal / Book Title: PLoS One
Volume: 15
Issue: 3
Copyright Statement: © 2020 Broadbent et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor/Funder: Wellcome Trust
Funder's Grant Number: 108818/Z/15/Z and 108818/Z/15/
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SYNCYTIAL VIRUS-INFECTION
Cell Differentiation
Cell Line
Child
Culture Media
Epithelial Cells
Goblet Cells
Humans
Nose
Primary Cell Culture
Respiratory Syncytial Viruses
Nose
Goblet Cells
Cell Line
Epithelial Cells
Humans
Respiratory Syncytial Viruses
Culture Media
Cell Differentiation
Child
Primary Cell Culture
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SYNCYTIAL VIRUS-INFECTION
General Science & Technology
Publication Status: Published
Article Number: ARTN e0228229
Online Publication Date: 2020-03-26
Appears in Collections:National Heart and Lung Institute



This item is licensed under a Creative Commons License Creative Commons