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Membrane protein mediated bilayer communication in networks of droplet interface bilayers

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Title: Membrane protein mediated bilayer communication in networks of droplet interface bilayers
Authors: Haylock, S
Friddin, M
Hindley, J
Rodriguez, E
Charalambous, K
Booth, P
Barter, L
Ces, O
Item Type: Journal Article
Abstract: Droplet interface bilayers (DIBs) are model membranes formed between lipid monolayer-encased water droplets in oil. Compared to conventional methods, one of the most unique properties of DIBs is that they can be connected together to generate multi-layered ‘tissue-like’ networks, however introducing communication pathways between these compartments typically relies on water-soluble pores that are unable to gate. Here, we show that network connectivity can instead be achieved using a water-insoluble membrane protein by successfully reconstituting a chemically activatable mutant of the mechanosensitive channel MscL into a network of DIBs. Moreover, we also show how the small molecule activator can diffuse through an open channel and across the neighbouring droplet to activate MscL present in an adjacent bilayer. This demonstration of membrane protein mediated bilayer communication could prove key toward developing the next generation of responsive bilayer networks capable of defining information flow inside a minimal tissue.
Issue Date: 12-Jun-2020
Date of Acceptance: 14-May-2020
URI: http://hdl.handle.net/10044/1/80477
DOI: 10.1038/s42004-020-0322-1
ISSN: 2399-3669
Publisher: Nature Research (part of Springer Nature)
Journal / Book Title: Communications Chemistry
Volume: 3
Copyright Statement: Copyright © the authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (E
Funder's Grant Number: BB/F013167/1
EP/G00465X/1
EP/J017566/1
CHBBC_P81995
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
MECHANOSENSITIVE CHANNEL
GATING MECHANISM
SINGLE RESIDUE
MSCL
TRANSPORTER
SYSTEMS
Publication Status: Published
Article Number: ARTN 77
Online Publication Date: 2020-06-12
Appears in Collections:Bioengineering
Chemistry
Biological and Biophysical Chemistry
Dyson School of Design Engineering
Faculty of Natural Sciences
Faculty of Engineering