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The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature

Title: The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature
Authors: Ciges-Tomas, JR
Alite, C
Humphrey, S
Donderisl, J
Bowring, J
Salvatella, X
Penades, JR
Marina, A
Item Type: Journal Article
Abstract: Stl is a master repressor encoded by Staphylococcus aureus pathogenicity islands (SaPIs) that maintains integration of these elements in the bacterial chromosome. After infection or induction of a resident helper phage, SaPIs are de-repressed by specific interactions of phage proteins with Stl. SaPIs have evolved a fascinating mechanism to ensure their promiscuous transfer by targeting structurally unrelated proteins performing identically conserved functions for the phage. Here we decipher the molecular mechanism of this elegant strategy by determining the structure of SaPIbov1 Stl alone and in complex with two structurally unrelated dUTPases from different S. aureus phages. Remarkably, SaPIbov1 Stl has evolved different domains implicated in DNA and partner recognition specificity. This work presents the solved structure of a SaPI repressor protein and the discovery of a modular repressor that acquires multispecificity through domain recruiting. Our results establish the mechanism that allows widespread dissemination of SaPIs in nature.
Issue Date: 15-Aug-2019
Date of Acceptance: 17-Jul-2019
URI: http://hdl.handle.net/10044/1/80422
DOI: 10.1038/s41467-019-11504-2
ISSN: 2041-1723
Publisher: Nature Research (part of Springer Nature)
Journal / Book Title: Nature Communications
Volume: 10
Copyright Statement: © 2019 The Author(s). This article is licensed under a Creative CommonsAttribution 4.0 International License, which permits use, sharing,adaptation, distribution and reproduction in any medium or format, as long as you giveappropriate credit to the original author(s) and the source, provide a link to the CreativeCommons license, and indicate if changes were made. The images or other third partymaterial in this article are included in the article’s Creative Commons license, unlessindicated otherwise in a credit line to the material. If material is not included in thearticle’s Creative Commons license and your intended use is not permitted by statutoryregulation or exceeds the permitted use, you will need to obtain permission directly fromthe copyright holder. To view a copy of this license, visithttp://creativecommons.org/licenses/by/4.0/.
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
DNA-BINDING DOMAINS
CATALYTIC MECHANISM
DUTPASES
REPLICATION
EXPRESSION
INDUCTION
PROTEINS
SYSTEM
Biological Coevolution
Crystallography, X-Ray
DNA-Binding Proteins
Genomic Islands
Models, Molecular
Staphylococcus Phages
Staphylococcus aureus
Staphylococcus aureus
Staphylococcus Phages
DNA-Binding Proteins
Crystallography, X-Ray
Genomic Islands
Models, Molecular
Biological Coevolution
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
DNA-BINDING DOMAINS
CATALYTIC MECHANISM
DUTPASES
REPLICATION
EXPRESSION
INDUCTION
PROTEINS
SYSTEM
Publication Status: Published
Article Number: ARTN 3676
Appears in Collections:Department of Infectious Diseases