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A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae

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Title: A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae
Authors: Simoni, A
Hammond, AM
Beaghton, AK
Galizi, R
Taxiarchi, C
Kyrou, K
Meacci, D
Gribble, M
Morselli, G
Burt, A
Nolan, T
Crisanti, A
Item Type: Journal Article
Abstract: Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce unisex populations that eventually collapse, but the underlying molecular mechanisms are unknown. We report a male-biased sex-distorter gene drive (SDGD) in the human malaria vector Anopheles gambiae. We induced super-Mendelian inheritance of the X-chromosome-shredding I-PpoI nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequence of the doublesex (dsx) gene. In modeling of invasion dynamics, SDGD was predicted to have a quicker impact on female mosquito populations than previously developed gene drives targeting female fertility. The SDGD at the dsx locus led to a male-only population from a 2.5% starting allelic frequency in 10-14 generations, with population collapse and no selection for resistance. Our results support the use of SDGD for malaria vector control.
Issue Date: 1-Sep-2020
Date of Acceptance: 1-Apr-2020
URI: http://hdl.handle.net/10044/1/79258
DOI: 10.1038/s41587-020-0508-1
ISSN: 1087-0156
Publisher: Nature Research
Start Page: 1054
End Page: 1060
Journal / Book Title: Nature Biotechnology
Volume: 38
Copyright Statement: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons. org/licenses/by/4.0/.
Sponsor/Funder: Bill & Melinda Gates Foundation
Silicon Valley Community Foundation
Funder's Grant Number: OPP1141988
Keywords: Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Publication Status: Published
Conference Place: United States
Online Publication Date: 2020-05-11
Appears in Collections:Faculty of Natural Sciences